The Alcohol Dehydrogenase Gene Adh1 is Induced in Aspergillus Flavus Grown on Medium Conducive to Aflatoxin Biosynthesis

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1994 Feb 1

PMID 8135521

Citations 15

Authors

C P Woloshuk

G A Payne

Affiliations

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Abstract

An Aspergillus flavus cDNA library was screened by differential hybridization to isolate clones corresponding to genes that are actively transcribed under culture conditions conducive to aflatoxin biosynthesis. One clone with a 1.28-kb insert was isolated, and its nucleotide sequence was determined. The nucleotide sequence of this clone had 75% DNA identity to those of the alcohol dehydrogenase genes from Aspergillus nidulans, and the putative polypeptide translated from the cDNA sequence had 82% similarity with the amino acid sequences of the A. nidulans proteins. Thus, this gene has been designated adh1. Southern hybridization analysis of genomic DNA from A. flavus indicated that there was one copy of the adh1 gene. Northern (RNA) hybridization analysis indicated that the adh1 transcript accumulated in culture medium conducive to aflatoxin production and the timing of accumulation of adh1 transcripts was similar to that for aflatoxin. Fusion of the promoter region of adh1 to a beta-glucuronidase reporter gene indicated that accumulation of the adh1 transcript was the result of transcriptional activation. These molecular data support previous physiological evidence that showed the importance of carbohydrate metabolism during aflatoxin biosynthesis.

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References

Skory C, CHANG P, Linz J . Regulated expression of the nor-1 and ver-1 genes associated with aflatoxin biosynthesis. Appl Environ Microbiol. 1993; 59(5):1642-6. PMC: 182131. DOI: 10.1128/aem.59.5.1642-1646.1993. View

Jornvall H, Eklund H, Branden C . Subunit conformation of yeast alcohol dehydrogenase. J Biol Chem. 1978; 253(23):8414-9. View

Dutton M . Enzymes and aflatoxin biosynthesis. Microbiol Rev. 1988; 52(2):274-95. PMC: 373139. DOI: 10.1128/mr.52.2.274-295.1988. View

CHANG P, Skory C, Linz J . Cloning of a gene associated with aflatoxin B1 biosynthesis in Aspergillus parasiticus. Curr Genet. 1992; 21(3):231-3. DOI: 10.1007/BF00336846. View

Payne G, Nystrom G, Bhatnagar D, Cleveland T, Woloshuk C . Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus. Appl Environ Microbiol. 1993; 59(1):156-62. PMC: 202071. DOI: 10.1128/aem.59.1.156-162.1993. View

Feng G, Chu F, Leonard T . Molecular cloning of genes related to aflatoxin biosynthesis by differential screening. Appl Environ Microbiol. 1992; 58(2):455-60. PMC: 195269. DOI: 10.1128/aem.58.2.455-460.1992. View

McKnight G, Kato H, Upshall A, Parker M, Saari G, OHara P . Identification and molecular analysis of a third Aspergillus nidulans alcohol dehydrogenase gene. EMBO J. 1985; 4(8):2093-9. PMC: 554467. DOI: 10.1002/j.1460-2075.1985.tb03897.x. View

Yamamoto Y, Taylor C, Acedo G, Cheng C, Conkling M . Characterization of cis-acting sequences regulating root-specific gene expression in tobacco. Plant Cell. 1991; 3(4):371-82. PMC: 160007. DOI: 10.1105/tpc.3.4.371. View

Luchese R, HARRIGAN W . Biosynthesis of aflatoxin--the role of nutritional factors. J Appl Bacteriol. 1993; 74(1):5-14. DOI: 10.1111/j.1365-2672.1993.tb02989.x. View

10.

Williamson J, Galili G, Larkins B, Gelvin S . The synthesis of a 19 kilodalton zein protein in transgenic petunia plants. Plant Physiol. 1988; 88(4):1002-7. PMC: 1055705. DOI: 10.1104/pp.88.4.1002. View

11.

Skory C, CHANG P, Cary J, Linz J . Isolation and characterization of a gene from Aspergillus parasiticus associated with the conversion of versicolorin A to sterigmatocystin in aflatoxin biosynthesis. Appl Environ Microbiol. 1992; 58(11):3527-37. PMC: 183140. DOI: 10.1128/aem.58.11.3527-3537.1992. View

12.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

13.

Woloshuk C, Seip E, Payne G, Adkins C . Genetic transformation system for the aflatoxin-producing fungus Aspergillus flavus. Appl Environ Microbiol. 1989; 55(1):86-90. PMC: 184058. DOI: 10.1128/aem.55.1.86-90.1989. View

14.

Yu J, Cary J, Bhatnagar D, Cleveland T, Keller N, Chu F . Cloning and characterization of a cDNA from Aspergillus parasiticus encoding an O-methyltransferase involved in aflatoxin biosynthesis. Appl Environ Microbiol. 1993; 59(11):3564-71. PMC: 182499. DOI: 10.1128/aem.59.11.3564-3571.1993. View

15.

Lockington R, Sealy-Lewis H, Scazzocchio C, Davies R . Cloning and characterization of the ethanol utilization regulon in Aspergillus nidulans. Gene. 1985; 33(2):137-49. DOI: 10.1016/0378-1119(85)90088-5. View

16.

Skory C, Horng J, Pestka J, Linz J . Transformation of Aspergillus parasiticus with a homologous gene (pyrG) involved in pyrimidine biosynthesis. Appl Environ Microbiol. 1990; 56(11):3315-20. PMC: 184948. DOI: 10.1128/aem.56.11.3315-3320.1990. View

17.

Gwynne D, Buxton F, Sibley S, Davies R, Lockington R, Scazzocchio C . Comparison of the cis-acting control regions of two coordinately controlled genes involved in ethanol utilization in Aspergillus nidulans. Gene. 1987; 51(2-3):205-16. DOI: 10.1016/0378-1119(87)90309-x. View

18.

Bennetzen J, Hall B . The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase. J Biol Chem. 1982; 257(6):3018-25. View

19.

Seip E, Woloshuk C, Payne G, Curtis S . Isolation and sequence analysis of a beta-tubulin gene from Aspergillus flavus and its use as a selectable marker. Appl Environ Microbiol. 1990; 56(12):3686-92. PMC: 185052. DOI: 10.1128/aem.56.12.3686-3692.1990. View

20.

Buchanan R, Jones S, Gerasimowicz W, Zaika L, Stahl H, Ocker L . Regulation of aflatoxin biosynthesis: assessment of the role of cellular energy status as a regulator of the induction of aflatoxin production. Appl Environ Microbiol. 1987; 53(6):1224-31. PMC: 203845. DOI: 10.1128/aem.53.6.1224-1231.1987. View