Binding of Paracoccidioides Brasiliensis to Laminin Through Surface Glycoprotein Gp43 Leads to Enhancement of Fungal Pathogenesis

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1994 Apr 1

PMID 8132354

Citations 68

Authors

A P Vicentini

J L Gesztesi

M F Franco

W De Souza

J Z de Moraes

L R Travassos

J D Lopes

Affiliations

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Abstract

Extracellular matrix protein laminin binds specifically to yeast forms of Paracoccidioides brasiliensis and enhances adhesion of the fungus to the surface of epithelial Madin-Darby canine kidney cells in vitro. Immunoblotting of fungal extracts showed that the gp43 glycoprotein is responsible for adhesion. This was confirmed by binding assays using purified gp43, with a Kd of 3.7 nM. The coating of P. brasiliensis yeast forms with laminin before injection into hamster testicles enhanced the fungus virulence, resulting in a faster and more severe granulomatous disease. These results indicate that interaction of fungi with extracellular matrix elements may constitute a basis for the evolution of fungal infection toward regional spreading and dissemination.

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