Fine Filaments in Lymphatic Endothelial Cells

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1976 Jan 1

PMID 812873

Citations 6

Authors

J M LAUWERYNS

J Baert

W DE LOECKER

Affiliations

Soon will be listed here.

Abstract

Several and various types of cells contain fine cytoplasmic filaments closely resembling the myofilaments of muscle cells (2, 18, 23, 24). In many of these cells and especially when cultured, it has been demonstrated that some of these filaments react with heavy meromyosin (HMM) in the same way as do the actin filaments of muscle cells (3, 6 7). This suggests that these filaments may be actinoid and form part of a contractile system. As fine intracytoplasmic filaments do occur in lymphatic endothelial cells (2, 14), we undertook an electron microscope investigation of their fine structure and their reaction on incubation with HMM and EDTA. We postulated that lymphatic endothelial cells possess a contractile filamentous system to which these filaments belong.

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References

GRAENICHER D, PORTZEHL H . THE INFLUENCE OF MAGNESIUM AND CALCIUM PYROPHOSPHATE CHELATES, OF FREE MAGNESIUM IONS, FREE CALCIUM IONS, AND FREE PYROPHOSPHATE IONS ON THE DISSOCIATION OF ACTYMYOSIN IN SOLUTION. Biochim Biophys Acta. 1964; 86:567-78. DOI: 10.1016/0304-4165(64)90096-0. View

Leak L, Burke J . Fine structure of the lymphatic capillary and the adjoining connective tissue area. Am J Anat. 1966; 118(3):785-809. DOI: 10.1002/aja.1001180308. View

Reynolds E . The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963; 17:208-12. PMC: 2106263. DOI: 10.1083/jcb.17.1.208. View

LOWEY S, Cohen C . Studies on the structure of myosin. J Mol Biol. 1962; 4:293-308. DOI: 10.1016/s0022-2836(62)80007-2. View

Kristensen B, Simonsen L, Pape L . Actin-like filaments in Ehrlich ascites tumor cells and their reaction with heavy meromyosin. Virchows Arch B Cell Pathol. 1973; 13(2):103-12. DOI: 10.1007/BF02889301. View

PANNER B, HONIG C . Filament ultrastructure and organization in vertebrate smooth muscle. Contraction hypothesis based on localization of actin and myosin. J Cell Biol. 1967; 35(2):303-21. PMC: 2107132. DOI: 10.1083/jcb.35.2.303. View

Kristensen B, Nielsen L . A two-filament system and interaction of heavy meromyosin (HMM) with thin filaments in smooth muscle. Z Zellforsch Mikrosk Anat. 1971; 122(3):350-6. DOI: 10.1007/BF00935994. View

LAUWERYNS J, Boussauw L . The ultrastructure of pulmonary lymphatic capillaries of newborn rabbits and of human infants. Lymphology. 1969; 2(3):108-29. View

Allison A, Davies P, de Petris S . Role of contractile microfilaments in macrophage movement and endocytosis. Nat New Biol. 1971; 232(31):153-5. DOI: 10.1038/newbio232153a0. View

10.

Burton P, Kirkland W . Actin detected in mouse neuroblastoma cells by binding of heavy meromyosin. Nat New Biol. 1972; 239(95):244-6. DOI: 10.1038/newbio239244a0. View

11.

Cooke P, Fay F . Correlation between fiber length, ultrastructure, and the length-tension relationship of mammalian smooth muscle. J Cell Biol. 1972; 52(1):105-16. PMC: 2108683. DOI: 10.1083/jcb.52.1.105. View

12.

LAUWERYNS J, Baert J . The role of the pulmonary lymphatics in the defenses of the distal lung: morphological and experimental studies of the transport mechanisms of intratracheally instillated particles. Ann N Y Acad Sci. 1974; 221:244-75. DOI: 10.1111/j.1749-6632.1974.tb28225.x. View

13.

Chang C, Goldman R . The localization of actin-like fibers in cultured neuroblastoma cells as revealed by heavy meromyosin binding. J Cell Biol. 1973; 57(3):867-74. PMC: 2109000. DOI: 10.1083/jcb.57.3.867. View

14.

Orci L, GABBAY K, Malaisse W . Pancreatic beta-cell web: its possible role in insulin secretion. Science. 1972; 175(4026):1128-30. DOI: 10.1126/science.175.4026.1128. View

15.

LAUWERYNS J, Boussauw L . The ultrastructure of lymphatic valves in the adult rabbit lung. Z Zellforsch Mikrosk Anat. 1973; 143(2):149-68. DOI: 10.1007/BF00307476. View

16.

Vajda J, Tomcsik M . The structure of the valves of the lymphatic vessels. Acta Anat (Basel). 1971; 78(4):521-31. DOI: 10.1159/000143611. View

17.

Leak L . Electron microscopic observations on lymphatic capillaries and the structural components of the connective tissue-lymph interface. Microvasc Res. 1970; 2(4):361-91. DOI: 10.1016/0026-2862(70)90031-2. View

18.

Ishikawa H, Bischoff R, Holtzer H . Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J Cell Biol. 1969; 43(2):312-28. PMC: 2107852. View

19.

Phelps P, LUFT J . Electron microscopical study of relaxation and constriction in frog arterioles. Am J Anat. 1969; 125(4):399-427. DOI: 10.1002/aja.1001250404. View

20.

Rohlich P, Olah I . Cross-striated fibrils in the endothelium of the rat myometral arterioles. J Ultrastruct Res. 1967; 18(5):667-76. DOI: 10.1016/s0022-5320(67)80212-0. View