An Antimicrobial Effect Associated with Rabbit Primary Aqueous Humour

Overview

Journal Br J Ophthalmol

Specialty Ophthalmology

Date 1994 Feb 1

PMID 8123624

Citations 3

Authors

J P Diamond

J P Leeming

A D Smart

P Duffus

A J Hedges

D L Easty

Affiliations

Soon will be listed here.

Abstract

Viability curves were recorded for a variety of bacteria suspended in rabbit primary aqueous humour. Controls included neat rabbit serum, dilute rabbit serum (1/50), dilute heat treated fetal bovine serum (1/50), and balanced salt solution. Rabbit aqueous humour was bactericidal in vitro for Staphylococcus aureus and Pseudomonas aeruginosa. Micrococcus spp, Streptococcus pneumoniae and Escherichia coli were not affected. The factor mediating the bactericidal effect survives filtration (0.2 micron porosity) and freezing in liquid nitrogen (-196 degrees C) but is labile when stored in air at temperatures of 4 degrees C, 25 degrees C, and 37 degrees C for 1 hour. The bactericidal effect was stable when aqueous humour was stored in an atmosphere of 5% CO2 at 37 degrees C for 3 hours. The antibacterial component in rabbit aqueous humour has yet to be identified.

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References

Shockley R, Jay W, Fishman P, Aziz M, Rissing J . Effect of inoculum size on the induction of endophthalmitis in aphakic rabbit eyes. Acta Ophthalmol (Copenh). 1985; 63(1):35-8. DOI: 10.1111/j.1755-3768.1985.tb05212.x. View

Heaven C, Mann P, Boase D . Endophthalmitis following extracapsular cataract surgery: a review of 32 cases. Br J Ophthalmol. 1992; 76(7):419-23. PMC: 504303. DOI: 10.1136/bjo.76.7.419. View

Mondino B, Rao H . Hemolytic complement activity in aqueous humor. Arch Ophthalmol. 1983; 101(3):465-8. DOI: 10.1001/archopht.1983.01040010465028. View

Tripathi R, Millard C, Tripathi B . Protein composition of human aqueous humor: SDS-PAGE analysis of surgical and post-mortem samples. Exp Eye Res. 1989; 48(1):117-30. DOI: 10.1016/0014-4835(89)90025-0. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Dickey J, K D Thompson , Jay W . Anterior chamber aspirate cultures after uncomplicated cataract surgery. Am J Ophthalmol. 1991; 112(3):278-82. DOI: 10.1016/s0002-9394(14)76728-5. View

Kimbrell D . Insect antibacterial proteins: not just for insects and against bacteria. Bioessays. 1991; 13(12):657-63. DOI: 10.1002/bies.950131207. View

Knisely T, Bleicher P, Vibbard C, Granstein R . Production of latent transforming growth factor-beta and other inhibitory factors by cultured murine iris and ciliary body cells. Curr Eye Res. 1991; 10(8):761-71. DOI: 10.3109/02713689109013870. View

MAURICE D . Protein dynamics in the eye studied with labelled proteins. Am J Ophthalmol. 1959; 47(1 Pt 2):361-8. DOI: 10.1016/s0002-9394(14)78042-0. View

10.

Forster R, Zachary I, Cottingham Jr A, Norton E . Further observations on the diagnosis cause, and treatment of endophthalmitis. Am J Ophthalmol. 1976; 81(1):52-6. DOI: 10.1016/0002-9394(76)90190-2. View

11.

Johnson J, Lott H . Standardization of the Coomassie Blue method for cerebrospinal fluid proteins. Clin Chem. 1978; 24(11):1931-3. View

12.

Burns R . Postoperative infections in an ophthalmologic hospital with comments upon bacteriophage typing of staphylococci as a preventative tool. Am J Ophthalmol. 1959; 48:519-26. View

13.

Dethlefs B . Experimental Klebsiella-induced endophthalmitis in the rabbit. Arch Ophthalmol. 1982; 100(12):1959-63. DOI: 10.1001/archopht.1982.01030040939015. View

14.

Yu T, Okamura R . Quantitative study of characteristic aqueous humor transferrin, serum transferrin and desialized serum transferrin in aqueous humor. Jpn J Ophthalmol. 1988; 32(3):268-74. View

15.

Jenkins C, McDonnell P, Spalton D . Randomised single blind trial to compare the toxicity of subconjunctival gentamicin and cefuroxime in cataract surgery. Br J Ophthalmol. 1990; 74(12):734-8. PMC: 1042278. DOI: 10.1136/bjo.74.12.734. View

16.

Sen D, SARIN G, Saha K . Immunoglobulins in human aqueous humour. Br J Ophthalmol. 1977; 61(3):216-7. PMC: 1042917. DOI: 10.1136/bjo.61.3.216. View

17.

Beyer T, Vogler G, Sharma D, ODonnell Jr F . Protective barrier effect of the posterior lens capsule in exogenous bacterial endophthalmitis--an experimental primate study. Invest Ophthalmol Vis Sci. 1984; 25(1):108-12. View

18.

Brote L, Gillquist J, Tarnvik A . Wound infections in general surgery. Wound contamination, rates of infection and some consequences. Acta Chir Scand. 1976; 142(2):99-106. View

19.

Bode Jr D, Gelender H, Forster R . A retrospective review of endophthalmitis due to coagulase-negative staphylococci. Br J Ophthalmol. 1985; 69(12):915-9. PMC: 1040882. DOI: 10.1136/bjo.69.12.915. View

20.

Davey P, Barza M, Peckman C . Spontaneous inhibition of bacterial growth in experimental gram-negative endophthalmitis. Invest Ophthalmol Vis Sci. 1987; 28(5):867-73. View