Characteristics of Adherence of Actinobacillus Actinomycetemcomitans to Epithelial Cells

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1994 Mar 1

PMID 8112865

Citations 18

Authors

D H Meyer

Affiliations

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Abstract

Actinobacillus actinomycetemcomitans smooth variants [SUNY 75(S), SUNY 465, 652] were investigated for their ability to adhere to KB epithelial cells. Both the type of medium (broth versus agar) and anaerobicity influenced adherence levels and cell surface characteristics. Optimal adherence was observed with all three strains after growth of the bacterial cells in broth under anaerobic conditions, a condition which was associated with extracellular microvesicles. Adherence of SUNY 75(S) also was correlated with extracellular amorphous material, whereas adherence of SUNY 465 was also associated with fimbriation which accompanied a smooth to rough phenotype shift. The relationship between adherence and extracellular vesicles, extracellular amorphous material, and fimbriation suggests that all of these components may function in A. actinomycetemcomitans adherence to epithelial cells. The phenotype shift observed in SUNY 465 cells is further evidence that A. actinomycetemcomitans SUNY 465 is predisposed to variant shifts which are associated with changes in adherence and invasion properties.

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References

Jones G, Freter R . Adhesive properties of Vibrio cholerae: nature of the interaction with isolated rabbit brush border membranes and human erythrocytes. Infect Immun. 1976; 14(1):240-5. PMC: 420869. DOI: 10.1128/iai.14.1.240-245.1976. View

Meyer D . Evidence that extracellular components function in adherence of Actinobacillus actinomycetemcomitans to epithelial cells. Infect Immun. 1993; 61(11):4933-6. PMC: 281260. DOI: 10.1128/iai.61.11.4933-4936.1993. View

Elder B, Boraker D . Whole-bacterial cell enzyme-linked immunosorbent assay for Streptococcus sanguis fimbrial antigens. J Clin Microbiol. 1982; 16(1):141-4. PMC: 272310. DOI: 10.1128/jcm.16.1.141-144.1982. View

Saglie F, Carranza Jr F, Newman M, Cheng L, Lewin K . Identification of tissue-invading bacteria in human periodontal disease. J Periodontal Res. 1982; 17(5):452-5. DOI: 10.1111/j.1600-0765.1982.tb02024.x. View

Gibbons R, Etherden I . Comparative hydrophobicities of oral bacteria and their adherence to salivary pellicles. Infect Immun. 1983; 41(3):1190-6. PMC: 264625. DOI: 10.1128/iai.41.3.1190-1196.1983. View

Kagermeier A, London J . Actinobacillus actinomycetemcomitans strains Y4 and N27 adhere to hydroxyapatite by distinctive mechanisms. Infect Immun. 1985; 47(3):654-8. PMC: 261347. DOI: 10.1128/iai.47.3.654-658.1985. View

Zambon J . Actinobacillus actinomycetemcomitans in human periodontal disease. J Clin Periodontol. 1985; 12(1):1-20. DOI: 10.1111/j.1600-051x.1985.tb01348.x. View

Weiss E, London J, Kolenbrander P, Kagermeier A, ANDERSEN R . Characterization of lectinlike surface components on Capnocytophaga ochracea ATCC 33596 that mediate coaggregation with gram-positive oral bacteria. Infect Immun. 1987; 55(5):1198-202. PMC: 260490. DOI: 10.1128/iai.55.5.1198-1202.1987. View

Sato H, Okinaga K . Role of pili in the adherence of Pseudomonas aeruginosa to mouse epidermal cells. Infect Immun. 1987; 55(8):1774-8. PMC: 260600. DOI: 10.1128/iai.55.8.1774-1778.1987. View

10.

Scannapieco F, Millar S, Reynolds H, Zambon J, LeVine M . Effect of anaerobiosis on the surface ultrastructure and surface proteins of Actinobacillus actinomycetemcomitans (Haemophilus actinomycetemcomitans). Infect Immun. 1987; 55(9):2320-3. PMC: 260702. DOI: 10.1128/iai.55.9.2320-2323.1987. View

11.

Kozlovsky A, Metzger Z, Eli I . Cell surface hydrophobicity of Actinobacillus actinomycetemcomitans Y4. J Clin Periodontol. 1987; 14(6):370-2. DOI: 10.1111/j.1600-051x.1987.tb00986.x. View

12.

Slots J, Listgarten M . Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal diseases. J Clin Periodontol. 1988; 15(2):85-93. DOI: 10.1111/j.1600-051x.1988.tb00999.x. View

13.

Murray P, Kern D, Winkler J . Identification of a galactose-binding lectin on Fusobacterium nucleatum FN-2. Infect Immun. 1988; 56(5):1314-9. PMC: 259818. DOI: 10.1128/iai.56.5.1314-1319.1988. View

14.

Rosan B, Slots J, Lamont R, Listgarten M, Nelson G . Actinobacillus actinomycetemcomitans fimbriae. Oral Microbiol Immunol. 1988; 3(2):58-63. DOI: 10.1111/j.1399-302x.1988.tb00082.x. View

15.

Preus H, Namork E, Olsen I . Fimbriation of Actinobacillus actinomycetemcomitans. Oral Microbiol Immunol. 1988; 3(2):93-4. DOI: 10.1111/j.1399-302x.1988.tb00090.x. View

16.

Sweet S, MacFarlane T, Samaranayake L . An in vitro method to study the adherence of oral bacteria to HeLa cells. Microbios. 1989; 60(242):15-22. View

17.

Singh U, Grenier D, McBride B . Bacteroides gingivalis vesicles mediate attachment of streptococci to serum-coated hydroxyapatite. Oral Microbiol Immunol. 1989; 4(4):199-203. DOI: 10.1111/j.1399-302x.1989.tb00252.x. View

18.

Inouye T, Ohta H, Kokeguchi S, Fukui K, Kato K . Colonial variation and fimbriation of Actinobacillus actinomycetemcomitans. FEMS Microbiol Lett. 1990; 57(1-2):13-7. DOI: 10.1016/0378-1097(90)90405-f. View

19.

St Geme 3rd J, Falkow S . Loss of capsule expression by Haemophilus influenzae type b results in enhanced adherence to and invasion of human cells. Infect Immun. 1991; 59(4):1325-33. PMC: 257846. DOI: 10.1128/iai.59.4.1325-1333.1991. View

20.

Wilson M . IgG antibody response of localized juvenile periodontitis patients to the 29 kilodalton outer membrane protein of Actinobacillus actinomycetemcomitans. J Periodontol. 1991; 62(3):211-8. DOI: 10.1902/jop.1991.62.3.211. View