The Lactate/pyruvate Shuttle in Spermatozoa: Operation in Vitro

Overview

Journal Arch Biochem Biophys

Publisher Elsevier

Specialties Biochemistry
Biophysics

Date 1994 Feb 1

PMID 8109982

Citations 13

Authors

F G Gallina

N M Gerez de Burgos

C Burgos

C E Coronel

A Blanco

Affiliations

Soon will be listed here.

Abstract

The operation of a shuttle for the transfer of reducing equivalents in the special mitochondria present in the middle piece of spermatozoa (sperm-type mitochondria, STM) was studied in reconstituted systems in vitro with mouse, rat, and rabbit STM. The redox couple lactate/pyruvate and the lactate dehydrogenase isozyme C4 are involved in the shuttle. It is active with rat and rabbit STM, while it does not work with mouse STM, probably because the influx of lactate into the mouse organelles is relatively poor. Ratios of consumption of pyruvate/lactate by STM were 21.6, 1.28, and 1.6 for mouse, rat, and rabbit organelles, respectively. The shuttle is inhibited by 0.6 mM mersalyl, a blocker of lactate transport. The operation of the shuttle would oxidize cytosolic NADH produced during aerobic glycolysis (or fructolysis) in spermatozoa of those species having an efficient lactate carrier in mitochondria.

Citing Articles

Pyruvate modulation of redox potential controls mouse sperm motility.

Schmidt C, Hale B, Bhowmick D, Miller W, Neufer P, Geyer C Dev Cell. 2023; 59(1):79-90.e6.

PMID: 38101411 PMC: 10872278. DOI: 10.1016/j.devcel.2023.11.011.

Linking paternally inherited mtDNA variants and sperm performance.

Bettinazzi S, Nadarajah S, Dalpe A, Milani L, Blier P, Breton S Philos Trans R Soc Lond B Biol Sci. 2019; 375(1790):20190177.

PMID: 31787040 PMC: 6939365. DOI: 10.1098/rstb.2019.0177.

Including the mitochondrial metabolism of L-lactate in cancer metabolic reprogramming.

de Bari L, Atlante A Cell Mol Life Sci. 2018; 75(15):2763-2776.

PMID: 29728715 PMC: 11105303. DOI: 10.1007/s00018-018-2831-y.

Differences in ATP Generation Via Glycolysis and Oxidative Phosphorylation and Relationships with Sperm Motility in Mouse Species.

Tourmente M, Villar-Moya P, Rial E, Roldan E J Biol Chem. 2015; 290(33):20613-26.

PMID: 26048989 PMC: 4536464. DOI: 10.1074/jbc.M115.664813.

Bioenergetics of mammalian sperm capacitation.

Ferramosca A, Zara V Biomed Res Int. 2014; 2014:902953.

PMID: 24791005 PMC: 3984864. DOI: 10.1155/2014/902953.