A Rapid and Sensitive Hemolysis Neutralization Assay for Palytoxin

Overview

Journal Toxicon

Specialty Toxicology

Date 1993 Jun 1

PMID 8102021

Citations 29

Authors

G S Bignami

Affiliations

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Abstract

The hemolytic action of palytoxin was exploited to develop a simple, sensitive assay with specificity based on a palytoxin neutralizing monoclonal antibody. Suspensions of murine erythrocytes incubated at 37 degrees C in round-bottom microtiter trays formed visible cell pellets which could be lysed by palytoxin. Hemolysis by palytoxin was time- and temperature-dependent, with a 24 hr detection limit of 1 pg/ml. The assay selectively detected palytoxin in a crude extract of Palythoa tuberculosa.

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