Baculovirus Expression of the Nucleocapsid Protein of a Puumala Serotype Hantavirus

Overview

Journal Virus Genes

Specialties Microbiology
Molecular Biology

Date 1994 Mar 1

PMID 8073635

Citations 3

Authors

C Schuldt

L Zoller

E K Bautz

G Darai

Affiliations

Soon will be listed here.

Abstract

Recombinant baculoviruses were generated harboring the entire coding region of the S segment cDNA of Hantavirus strain CG 18-20 that belongs to the Puumala serotype. The recombinant nucleocapsid protein was expressed in Sf9 cells and shown to be antigenically identical with the authentic viral nucleocapsid protein by means of immunoblot analysis. Acute-phase and convalescent sera from European HFRS patients recognized the recombinant nucleocapsid protein in Western blots and the recombinant Baculovirus in indirect immunofluorescence assays. Insect cells infected with the recombinant Baculoviruses proved to be a suitable noninfectious substitute for Hantavirus-infected Vero E6 cells as an antigen source for immunodiagnostic assays allowing the detection of antibodies in HFRS patients.

Citing Articles

Expression of the nucleoprotein of the Puumala virus from the recombinant Semliki Forest virus replicon: characterization and use as a potential diagnostic tool.

Billecocq A, Coudrier D, Boue F, Combes B, Zeller H, Artois M Clin Diagn Lab Immunol. 2003; 10(4):658-63.

PMID: 12853401 PMC: 164253. DOI: 10.1128/cdli.10.4.658-663.2003.

Expression of immunogenic Puumala virus nucleocapsid protein in transgenic tobacco and potato plants.

KEHM R, Jakob N, Welzel T, Tobiasch E, Viczian O, Jock S Virus Genes. 2001; 22(1):73-83.

PMID: 11210942 DOI: 10.1023/a:1008186403612.

Stable expression of nucleocapsid proteins of Puumala and Hantaan virus in mammalian cells.

Welzel T, KEHM R, Tidona C, Muranyi W, Darai G Virus Genes. 1998; 17(2):185-98.

PMID: 9857992 DOI: 10.1023/a:1008076926061.

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