Cell Lines Used for the Selection of Recombinant Baculovirus

Overview

Journal In Vitro Cell Dev Biol Anim

Publisher Springer

Specialties Biology
Cell Biology

Date 1994 Apr 1

PMID 8069452

Citations 2

Authors

J E Maruniak

J J Rodrigues

Affiliations

Soon will be listed here.

Abstract

Four insect cell lines were used to isolate two recombinant baculoviruses which had the beta-galactosidase (beta-gal) gene for colorimetric assay purposes. Plaque assays were performed using two Trichoplusia ni cell lines: BTI-TN-5B1-4 and TN-368, and two Spodptera frugiperda cell lines: IPLB-SF-21AE and SF9. The number of plaques (occlusion positive and blue beta-gal+ recombinants) formed in the Trichoplusia cells was higher than in the Spodoptera cells. The appearance of Autographa californica NPV polyhedra was also faster in the T. ni cell lines. The effect of cell passage on the plaque formation proved to be critical when two different passages of the SF9 cells were tested. The higher passage produced a lower viral titration. The size and time of appearance of the plaques was also different.

Citing Articles

Efficient production of foot-and-mouth disease virus empty capsids in insect cells following down regulation of 3C protease activity.

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Productivity of insect cells for recombinant proteins.

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