Ubiquitin and Ubiquitin-protein Conjugates in PC12h Cells: Changes During Neuronal Differentiation

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1994 Apr 1

PMID 8065495

Citations 6

Authors

K Takada

T Kanda

K Ohkawa

M Matsuda

Affiliations

Soon will be listed here.

Abstract

Ubiquitin and ubiquitin-protein conjugates in PC12h cells were detected with in vitro [125I]ubiquitination, and quantified by immunoblotting. These levels were altered by nerve growth factor (NGF), which promotes neuronal differentiation. (i) Levels of high molecular weight (HMW) ubiquitin-protein conjugates ranging from 40 to 1,000 kDa were increased by 2 days of NGF treatment, and remained high up to 10 days of NGF treatment. (ii) Ubiquitin and a 23-kDa conjugate tended to be decreased from days 2 to 10 of NGF treatment. 10-Day culture with 10 nM staurosporine, n protein kinase inhibitor, that blocks NGF-induced neurite outgrowth suppressed the NGF-induced increases in levels of HMW conjugates. Cyclic AMP and forskolin, both of which promote neurite outgrowth, mimicked the NGF-induced changes in ubiquitin and HMW conjugates, but phorbol ester and epidermal growth factor had little effect. These findings suggest that changes in ubiquitin-protein conjugates are closely coupled with neuronal differentiation.

Citing Articles

Ubiquitination detection techniques.

De Silva A, Page R Exp Biol Med (Maywood). 2023; 248(15):1333-1346.

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Real-time bio-layer interferometry ubiquitination assays as alternatives to western blotting.

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The effect of hypothalamic peptides, neurohormone C and proline-rich peptide-1on the Ca-handling system in heartin pathophysiological conditions.

Guevorkian A Heliyon. 2020; 6(6):e04360.

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NGF-Dependent Changes in Ubiquitin Homeostasis Trigger Early Cholinergic Degeneration in Cellular and Animal AD-Model.

Latina V, Caioli S, Zona C, Ciotti M, Borreca A, Calissano P Front Cell Neurosci. 2019; 12:487.

PMID: 30618634 PMC: 6300588. DOI: 10.3389/fncel.2018.00487.

Isolation of ubiquitin-E2 (ubiquitin-conjugating enzyme) complexes from erythroleukaemia cells using immunoaffinity techniques.

Takada K, Hirakawa T, Yokosawa H, Okawa Y, Taguchi H, Ohkawa K Biochem J. 2001; 356(Pt 1):199-206.

PMID: 11336652 PMC: 1221828. DOI: 10.1042/0264-6021:3560199.

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