Carcinoma of Stomach and Breast with Lymphoid Stroma: Localisation of Epstein-Barr Virus

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1994 Jun 1

PMID 8063937

Citations 27

Authors

K Horiuchi

K Mishima

M Ohsawa

K Aozasa

Affiliations

Soon will be listed here.

Abstract

Aims: To determine the presence of Epstein-Barr virus (EBV) genome in six patients (three with gastric and three with breast carcinoma) with severe small lymphoid cell infiltration.

Methods: The polymerase chain reaction and in situ hybridisation were used to detect EBV genome. The number and distribution of T and B lymphocytes were evaluated by immunohistochemistry.

Results: Histologically all of the patients had poorly differentiated tumours. Immunohistochemistry showed that T cells predominated in three cases, B cell in two, and almost equal numbers in one case. PCR showed that the EBV genome was present in two cases each of gastric and breast carcinoma. In situ hybridisation for EBV genome provided positive signals only in the small lymphoid cells in one gastric and two breast carcinomas giving a positive reaction for EBV genome by PCR. The gastric and breast cancer cells did not give positive signals.

Conclusions: Severe lymphoid cell infiltration in gastric and breast carcinoma does not necessarily indicate that these tumours are associated with EBV. Larger numbers of cases will need to be studied to confirm this.

Citing Articles

PD-L1 expression in EBV associated gastric cancer: a systematic review and meta-analysis.

Lima A, Sousa H, Medeiros R, Nobre A, Machado M Discov Oncol. 2022; 13(1):19.

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Identification of Novel Endogenous Controls for qPCR Normalization in SK-BR-3 Breast Cancer Cell Line.

Jain N, Mitre I, Nitisa D, Pirsko V, Cakstina-Dzerve I Genes (Basel). 2021; 12(10).

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Postoperative outcomes of gastric carcinoma with lymphoid stroma.

Iwasaki K, Suda T, Takano Y, Ohno Y, Yamada E, Okazaki N World J Surg Oncol. 2020; 18(1):102.

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[Clinicopathological and molecular characteristics of Epstein-Barr virus associated gastric cancer: a single center large sample case investigation].

Yang Y, Liu Y, Wang X, Ji K, Li Z, Bai J Beijing Da Xue Xue Bao Yi Xue Ban. 2019; 51(3):451-458.

PMID: 31209416 PMC: 7439042. DOI: 10.19723/j.issn.1671-167X.2019.03.012.

Poor prognosis in Epstein-Barr virus-negative gastric cancer with lymphoid stroma is associated with immune phenotype.

Cho C, Kang H, Ryu Y, Park Y, Jeong H, Park Y Gastric Cancer. 2018; 21(6):925-935.

PMID: 29627937 DOI: 10.1007/s10120-018-0820-3.

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