Nucleotide Sequence of the Wolinella Succinogenes Flagellin, Which Contains in the Antigenic Domain Two Conserved Regions Also Present in Campylobacter Spp. and Helicobacter Pylori

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1994 Aug 1

PMID 8051032

Citations 4

Authors

S C Schuster

M Bauer

J KELLERMANN

F Lottspeich

E Baeuerlein

Affiliations

Soon will be listed here.

Abstract

Wolinella succinogenes possesses one polar flagellum, which shows a characteristic surface pattern of parallel lines along the axis of the filament in electron microscopic images. We determined the gene sequence of the Wolinella flagellin, which is, as in most other bacteria, the only structural component of the filament. Sequence comparison with other members of the Proteobacteria revealed two highly conserved regions in the central part of the flagellin molecule among Campylobacter spp. and Helicobacter pylori, an area that had previously been described as highly variable. Similar surface patterns are found in related polarly flagellated bacteria, but not in Escherichia coli and Bacillus subtilis, which also lack these conserved regions.

Citing Articles

Complete genome sequence and analysis of Wolinella succinogenes.

Baar C, Eppinger M, Raddatz G, Simon J, Lanz C, Klimmek O Proc Natl Acad Sci U S A. 2003; 100(20):11690-5.

PMID: 14500908 PMC: 208819. DOI: 10.1073/pnas.1932838100.

Polymorphism of flagellin A gene in Helicobacter pylori.

Ji W, Hu J, Qiu J, Peng D, Shi B, Zhou S World J Gastroenterol. 2002; 7(6):783-7.

PMID: 11854901 PMC: 4695594. DOI: 10.3748/wjg.v7.i6.783.

Mutagenesis of Burkholderia pseudomallei with Tn5-OT182: isolation of motility mutants and molecular characterization of the flagellin structural gene.

Deshazer D, Brett P, Carlyon R, Woods D J Bacteriol. 1997; 179(7):2116-25.

PMID: 9079894 PMC: 178945. DOI: 10.1128/jb.179.7.2116-2125.1997.

Cloning, sequencing, and phenotypic analysis of laf1, encoding the flagellin of the lateral flagella of Azospirillum brasilense Sp7.

Moens S, Michiels K, Keijers V, Van Leuven F, Vanderleyden J J Bacteriol. 1995; 177(19):5419-26.

PMID: 7559324 PMC: 177346. DOI: 10.1128/jb.177.19.5419-5426.1995.

References

Morooka T, Umeda A, Amako K . Morphological differences in flagella in Campylobacter fetus subsp. intestinalis and C. fetus subsp. jejuni. Microbiol Immunol. 1983; 27(8):655-62. DOI: 10.1111/j.1348-0421.1983.tb00628.x. View

Wolin M, WOLIN E, Jacobs N . Cytochrome-producing anaerobic Vibrio succinogenes, sp. n. J Bacteriol. 1961; 81:911-7. PMC: 314759. DOI: 10.1128/jb.81.6.911-917.1961. View

Aizawa S, Dean G, Jones C, Macnab R, Yamaguchi S . Purification and characterization of the flagellar hook-basal body complex of Salmonella typhimurium. J Bacteriol. 1985; 161(3):836-49. PMC: 214974. DOI: 10.1128/jb.161.3.836-849.1985. View

Romaniuk P, Zoltowska B, Trust T, Lane D, Olsen G, Pace N . Campylobacter pylori, the spiral bacterium associated with human gastritis, is not a true Campylobacter sp. J Bacteriol. 1987; 169(5):2137-41. PMC: 212113. DOI: 10.1128/jb.169.5.2137-2141.1987. View

Henikoff S . Unidirectional digestion with exonuclease III in DNA sequence analysis. Methods Enzymol. 1987; 155:156-65. DOI: 10.1016/0076-6879(87)55014-5. View

Eckerskorn C, Mewes W, Goretzki H, Lottspeich F . A new siliconized-glass fiber as support for protein-chemical analysis of electroblotted proteins. Eur J Biochem. 1988; 176(3):509-19. DOI: 10.1111/j.1432-1033.1988.tb14308.x. View

Macnab R, DeRosier D . Bacterial flagellar structure and function. Can J Microbiol. 1988; 34(4):442-51. DOI: 10.1139/m88-077. View

Joys T . The flagellar filament protein. Can J Microbiol. 1988; 34(4):452-8. DOI: 10.1139/m88-078. View

Brock F, Murray R . The ultrastructure and ATPase nature of polar membrane in Campylobacter jejuni. Can J Microbiol. 1988; 34(5):594-604. DOI: 10.1139/m88-099. View

10.

Kupper J, Wildhaber I, Gao Z, Baeuerlein E . Basal-body-associated disks are additional structural elements of the flagellar apparatus isolated from Wolinella succinogenes. J Bacteriol. 1989; 171(5):2803-10. PMC: 209967. DOI: 10.1128/jb.171.5.2803-2810.1989. View

11.

Logan S, Trust T, Guerry P . Evidence for posttranslational modification and gene duplication of Campylobacter flagellin. J Bacteriol. 1989; 171(6):3031-8. PMC: 210011. DOI: 10.1128/jb.171.6.3031-3038.1989. View

12.

Nuijten P, Van Asten F, Gaastra W, van der Zeijst B . Structural and functional analysis of two Campylobacter jejuni flagellin genes. J Biol Chem. 1990; 265(29):17798-804. View

13.

Kostrzynska M, Betts J, Austin J, Trust T . Identification, characterization, and spatial localization of two flagellin species in Helicobacter pylori flagella. J Bacteriol. 1991; 173(3):937-46. PMC: 207209. DOI: 10.1128/jb.173.3.937-946.1991. View

14.

Kanto S, Okino H, Aizawa S, Yamaguchi S . Amino acids responsible for flagellar shape are distributed in terminal regions of flagellin. J Mol Biol. 1991; 219(3):471-80. DOI: 10.1016/0022-2836(91)90187-b. View

15.

Trachtenberg S, DeRosier D . A molecular switch: subunit rotations involved in the right-handed to left-handed transitions of Salmonella typhimurium flagellar filaments. J Mol Biol. 1991; 220(1):67-77. DOI: 10.1016/0022-2836(91)90381-f. View

16.

Guerry P, Alm R, Power M, Logan S, Trust T . Role of two flagellin genes in Campylobacter motility. J Bacteriol. 1991; 173(15):4757-64. PMC: 208154. DOI: 10.1128/jb.173.15.4757-4764.1991. View

17.

Schuster S, Baeuerlein E . Location of the basal disk and a ringlike cytoplasmic structure, two additional structures of the flagellar apparatus of Wolinella succinogenes. J Bacteriol. 1992; 174(1):263-8. PMC: 205704. DOI: 10.1128/jb.174.1.263-268.1992. View

18.

Lane D, HARRISON Jr A, Stahl D, Pace B, Giovannoni S, Olsen G . Evolutionary relationships among sulfur- and iron-oxidizing eubacteria. J Bacteriol. 1992; 174(1):269-78. PMC: 205705. DOI: 10.1128/jb.174.1.269-278.1992. View

19.

Leying H, Suerbaum S, Geis G, Haas R . Cloning and genetic characterization of a Helicobacter pylori flagellin gene. Mol Microbiol. 1992; 6(19):2863-74. DOI: 10.1111/j.1365-2958.1992.tb01466.x. View

20.

Khan S . Gene to ultrastructure: the case of the flagellar basal body. J Bacteriol. 1993; 175(8):2169-74. PMC: 204500. DOI: 10.1128/jb.175.8.2169-2174.1993. View