Expression of Sunflower Low-molecular-weight Heat-shock Proteins During Embryogenesis and Persistence After Germination: Localization and Possible Functional Implications

Overview

Journal Plant Mol Biol

Date 1994 Jun 1

PMID 8049372

Citations 46

Authors

M A Coca

C. Almoguera

J. Jordano

Affiliations

Soon will be listed here.

Abstract

We isolated and sequenced Ha hsp17.9, a DNA complementary (cDNA) of dry-seed stored mRNA that encodes a low-molecular-weight heat-shock protein (LMW HSP). Sequence analysis identified Ha hsp17.9, and the previously reported Ha hsp17.6, as cDNAs encoding proteins (HSP17.6 and HSP17.9) which belong to different families of cytoplasmic LMW HSPs. Using specific antibodies we observed differential expression of both proteins during zygotic embryogenesis under controlled environment, and a remarkable persistence of these LMW HSPs during germination. Immuno-blot analysis of HSP17.9 proteins in two-dimensional gels revealed that the polypeptides expressed in embryos were indistinguishable from LMW HSPs expressed in vegetative tissues in response to water deficit; but they appeared different from homologous proteins expressed in response to thermal-stress. Tissue-print immunolocalization experiments showed that HSP17.9 and HSP17.6 were homogeneously distributed in every tissue of desiccation-tolerant dry seeds and young seedlings under non-stress conditions. These results demonstrate developmental regulation of specific, cytoplasmic, plant LMW HSPs, suggesting also their involvement in water-stress tolerance.

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References

Lutcke H, Chow K, Mickel F, Moss K, Kern H, SCHEELE G . Selection of AUG initiation codons differs in plants and animals. EMBO J. 1987; 6(1):43-8. PMC: 553354. DOI: 10.1002/j.1460-2075.1987.tb04716.x. View

Raschke E, Baumann G, Schoffl F . Nucleotide sequence analysis of soybean small heat shock protein genes belonging to two different multigene families. J Mol Biol. 1988; 199(4):549-57. DOI: 10.1016/0022-2836(88)90300-2. View

Wang C, Lin B . The disappearance of an hsc70 species in mung bean seed during germination: purification and characterization of the protein. Plant Mol Biol. 1993; 21(2):317-29. DOI: 10.1007/BF00019947. View

Almoguera C, Jordano J . Developmental and environmental concurrent expression of sunflower dry-seed-stored low-molecular-weight heat-shock protein and Lea mRNAs. Plant Mol Biol. 1992; 19(5):781-92. DOI: 10.1007/BF00027074. View

Heikkila J . Heat shock gene expression and development. I. An overview of fungal, plant, and poikilothermic animal developmental systems. Dev Genet. 1993; 14(1):1-5. DOI: 10.1002/dvg.1020140102. View

Domoney C, Ellis N, Turner L, Casey R . A developmentally regulated early-embryogenesis protein in pea (Pisum sativum L.) is related to the heat-shock protein (HSP70) gene family. Planta. 2013; 184(3):350-5. DOI: 10.1007/BF00195336. View

Allen R, Nessler C, Thomas T . Developmental expression of sunflower 11S storage protein genes. Plant Mol Biol. 2013; 5(3):165-73. DOI: 10.1007/BF00015680. View

Lindquist S, Craig E . The heat-shock proteins. Annu Rev Genet. 1988; 22:631-77. DOI: 10.1146/annurev.ge.22.120188.003215. View

Bouchard R . Characterization of expressed meiotic prophase repeat transcript clones of Lilium: meiosis-specific expression, relatedness, and affinities to small heat shock protein genes. Genome. 1990; 33(1):68-79. DOI: 10.1139/g90-012. View

10.

Atkinson B, Raizada M, Bouchard R, Frappier R, Walden D . The independent stage-specific expression of the 18-kDa heat shock protein genes during microsporogenesis in Zea mays L. Dev Genet. 1993; 14(1):15-26. DOI: 10.1002/dvg.1020140104. View

11.

Kurtz S, Rossi J, Petko L, Lindquist S . An ancient developmental induction: heat-shock proteins induced in sporulation and oogenesis. Science. 1986; 231(4742):1154-7. DOI: 10.1126/science.3511530. View

12.

Ried J, Walker-Simmons M . Group 3 Late Embryogenesis Abundant Proteins in Desiccation-Tolerant Seedlings of Wheat (Triticum aestivum L.). Plant Physiol. 1993; 102(1):125-131. PMC: 158754. DOI: 10.1104/pp.102.1.125. View

13.

Jakob U, Gaestel M, Engel K, Buchner J . Small heat shock proteins are molecular chaperones. J Biol Chem. 1993; 268(3):1517-20. View

14.

Gyorgyey J, Gartner A, Nemeth K, Magyar Z, Hirt H, Heberle-Bors E . Alfalfa heat shock genes are differentially expressed during somatic embryogenesis. Plant Mol Biol. 1991; 16(6):999-1007. DOI: 10.1007/BF00016072. View

15.

Helm K, Abernethy R . Heat Shock Proteins and Their mRNAs in Dry and Early Imbibing Embryos of Wheat. Plant Physiol. 1990; 93(4):1626-33. PMC: 1062721. DOI: 10.1104/pp.93.4.1626. View

16.

Hernandez L, Vierling E . Expression of Low Molecular Weight Heat-Shock Proteins under Field Conditions. Plant Physiol. 1993; 101(4):1209-1216. PMC: 160641. DOI: 10.1104/pp.101.4.1209. View

17.

Duck N, McCormick S, Winter J . Heat shock protein hsp70 cognate gene expression in vegetative and reproductive organs of Lycopersicon esculentum. Proc Natl Acad Sci U S A. 1989; 86(10):3674-8. PMC: 287202. DOI: 10.1073/pnas.86.10.3674. View

18.

Lavoie J, Tanguay R, Landry J . Induction of Chinese hamster HSP27 gene expression in mouse cells confers resistance to heat shock. HSP27 stabilization of the microfilament organization. J Biol Chem. 1993; 268(5):3420-9. View

19.

Heikkila J . Heat shock gene expression and development. II. An overview of mammalian and avian developmental systems. Dev Genet. 1993; 14(2):87-91. DOI: 10.1002/dvg.1020140202. View

20.

Kruse E, Liu Z, Kloppstech K . Expression of heat shock proteins during development of barley. Plant Mol Biol. 1993; 23(1):111-22. DOI: 10.1007/BF00021424. View