Degradation of RRNA in Salmonella Strains: a Novel Mechanism to Regulate the Concentrations of RRNA and Ribosomes

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1994 Aug 1

PMID 8045909

Citations 30

Authors

D Hsu

L M Shih

Y C Zee

Affiliations

Soon will be listed here.

Abstract

We have previously shown that the 23S rRNA of Salmonella strains is highly fragmented by specific enzyme cleavages. In this article, we report that 23S rRNA of Salmonella strains is rapidly degraded as the cells enter the stationary phase. More than 90% of the 23S rRNA is degraded when the cells reach the stationary phase. The rate of degradation of 23S rRNA correlated with its degree of fragmentation. This degradation is probably mediated by newly synthesized protein factor(s), since treatment with chloramphenicol or rifampin inhibits the rRNA degradation. We propose that degradation of 23S rRNA is a novel mechanism in the regulation of the bacterial 23S rRNA and ribosome concentration and that this additional regulatory mechanism provides some selective advantage to cells.

Citing Articles

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References

Vicente M, Kushner S, Garrido T, Aldea M . The role of the 'gearbox' in the transcription of essential genes. Mol Microbiol. 1991; 5(9):2085-91. DOI: 10.1111/j.1365-2958.1991.tb02137.x. View

Zacharias M, Theissen G, Bradaczek C, Wagner R . Analysis of sequence elements important for the synthesis and control of ribosomal RNA in E coli. Biochimie. 1991; 73(6):699-712. DOI: 10.1016/0300-9084(91)90050-b. View

Flardh K, Cohen P, Kjelleberg S . Ribosomes exist in large excess over the apparent demand for protein synthesis during carbon starvation in marine Vibrio sp. strain CCUG 15956. J Bacteriol. 1992; 174(21):6780-8. PMC: 207353. DOI: 10.1128/jb.174.21.6780-6788.1992. View

Shine J, Dalgarno L . The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974; 71(4):1342-6. PMC: 388224. DOI: 10.1073/pnas.71.4.1342. View

Dennis P . In vivo stability, maturation and relative differential synthesis rates of individual ribosomal proteins in Escherichia coli B/r. J Mol Biol. 1974; 88(1):25-41. DOI: 10.1016/0022-2836(74)90293-9. View

Gausing K . Regulation of ribosome production in Escherichia coli: synthesis and stability of ribosomal RNA and of ribosomal protein messenger RNA at different growth rates. J Mol Biol. 1977; 115(3):335-54. DOI: 10.1016/0022-2836(77)90158-9. View

Gold L, Pribnow D, Schneider T, Shinedling S, Singer B, Stormo G . Translational initiation in prokaryotes. Annu Rev Microbiol. 1981; 35:365-403. DOI: 10.1146/annurev.mi.35.100181.002053. View

WITTMANN H . Components of bacterial ribosomes. Annu Rev Biochem. 1982; 51:155-83. DOI: 10.1146/annurev.bi.51.070182.001103. View

Nomura M, Gourse R, Baughman G . Regulation of the synthesis of ribosomes and ribosomal components. Annu Rev Biochem. 1984; 53:75-117. DOI: 10.1146/annurev.bi.53.070184.000451. View

10.

Lindahl L, Zengel J . Ribosomal genes in Escherichia coli. Annu Rev Genet. 1986; 20:297-326. DOI: 10.1146/annurev.ge.20.120186.001501. View

11.

Burgin A, Parodos K, Lane D, Pace N . The excision of intervening sequences from Salmonella 23S ribosomal RNA. Cell. 1990; 60(3):405-14. DOI: 10.1016/0092-8674(90)90592-3. View

12.

Wada A, Yamazaki Y, Fujita N, Ishihama A . Structure and probable genetic location of a "ribosome modulation factor" associated with 100S ribosomes in stationary-phase Escherichia coli cells. Proc Natl Acad Sci U S A. 1990; 87(7):2657-61. PMC: 53749. DOI: 10.1073/pnas.87.7.2657. View

13.

Hsu D, Pan M, Zee Y, Lefebvre R . Unique ribosome structure of Leptospira interrogans is composed of four rRNA components. J Bacteriol. 1990; 172(6):3478-80. PMC: 209161. DOI: 10.1128/jb.172.6.3478-3480.1990. View

14.

Verbeek H, Nilsson L, Baliko G, Bosch L . Potential binding sites of the trans-activator FIS are present upstream of all rRNA operons and of many but not all tRNA operons. Biochim Biophys Acta. 1990; 1050(1-3):302-6. DOI: 10.1016/0167-4781(90)90185-5. View

15.

Josaitis C, Gaal T, Ross W, Gourse R . Sequences upstream of the-35 hexamer of rrnB P1 affect promoter strength and upstream activation. Biochim Biophys Acta. 1990; 1050(1-3):307-11. DOI: 10.1016/0167-4781(90)90186-6. View

16.

Lange R . Identification of a central regulator of stationary-phase gene expression in Escherichia coli. Mol Microbiol. 1991; 5(1):49-59. DOI: 10.1111/j.1365-2958.1991.tb01825.x. View

17.

Hsu D, Zee Y, INGRAHAM J, Shih L . Diversity of cleavage patterns of Salmonella 23S rRNA. J Gen Microbiol. 1992; 138(1):199-203. DOI: 10.1099/00221287-138-1-199. View