Effects of Tri-iodothyronine Administration on the Disposal of Oral [1-14C]triolein, Lipoprotein Lipase Activity and Lipogenesis in the Rat During Lactation and on Removal of the Litter

Overview

Journal Biochem J

Specialty Biochemistry

Date 1994 Jul 15

PMID 8042994

Citations 1

Authors

M del Prado

T H Da Costa

D H Williamson

Affiliations

Soon will be listed here.

Abstract

The effect of tri-iodothyronine (T3) administration on the utilization of dietary [14C]lipid by the mammary gland and adipose tissue of lactating and litter-removed rats was studied. (1) After an oral load of [1-14C]triolein, the lactating rats treated with T3 (50 micrograms/100 g body wt.) over 24 h showed an increase in 14CO2 production and a decrease in the total [14C]lipid transferred through the mammary gland that was paralleled by a decrease in tissue lipoprotein lipase (LPL) activity. (2) T3 administration decreased plasma prolactin in the lactating rats. Prolactin replacement in T3-treated rats restored LPL activity in the mammary gland, but did not increase the amount of dietary [14C]lipid transferred to the milk. (3) Chronic T3 administration (4 days) to lactating rats did not affect pup growth or the lipogenic rate in the mammary gland. (4) The administration of T3 to litter-removed rats inhibited the increase of LPL activity in white adipose tissue and decreased the accumulation of dietary [14C]lipid. This decrease was accompanied by increased 14CO2 production and [14C]lipid accumulation in skeletal muscle and heart. (5) It is concluded that hyperthyroidism depresses LPL activity in mammary gland and white adipose tissue, but not in muscle. The increased accumulation of [14C]lipid in muscle and increased production of 14CO2 in lactating and in litter-removed rats treated with T3 is in part due to the decreased total LPL in mammary gland and adipose tissue respectively, which are therefore less able to compete with muscle for the available plasma triacylglycerols.

Citing Articles

Hyperthyroidism affects lipid metabolism in lactating and suckling rats.

Varas S, Jahn G, Gimenez M Lipids. 2001; 36(8):801-6.

PMID: 11592730 DOI: 10.1007/s11745-001-0787-4.

References

OPPENHEIMER J, Schwartz H, Lane J, Thompson M . Functional relationship of thyroid hormone-induced lipogenesis, lipolysis, and thermogenesis in the rat. J Clin Invest. 1991; 87(1):125-32. PMC: 295007. DOI: 10.1172/JCI114961. View

Blennemann B, Moon Y, Freake H . Tissue-specific regulation of fatty acid synthesis by thyroid hormone. Endocrinology. 1992; 130(2):637-43. DOI: 10.1210/endo.130.2.1733712. View

Saffari B, Ong J, Kern P . Regulation of adipose tissue lipoprotein lipase gene expression by thyroid hormone in rats. J Lipid Res. 1992; 33(2):241-9. View

Krug J, Lampeter E, Williams A, Procaccini E, Cartledge C, Signore A . Immunotherapy with ciamexon in the non obese diabetic (NOD) mouse. Horm Metab Res. 1992; 24(1):1-4. DOI: 10.1055/s-2007-1003240. View

Rosato R, Gimenez M, Jahn G . Effects of chronic thyroid hormone administration on pregnancy, lactogenesis and lactation in the rat. Acta Endocrinol (Copenh). 1992; 127(6):547-54. DOI: 10.1530/acta.0.1270547. View

Da Costa T, Williamson D . Effects of exogenous insulin or vanadate on disposal of dietary triacylglycerols between mammary gland and adipose tissue in the lactating rat: insulin resistance in white adipose tissue. Biochem J. 1993; 290 ( Pt 2):557-61. PMC: 1132310. DOI: 10.1042/bj2900557. View

Cruz M, Williamson D . Effects of triiodothyronine administration on dietary [14C]triolein partitioning between deposition in adipose tissue and oxidation to [14C]CO2 in ad libitum-fed or food-restricted rats. Biochim Biophys Acta. 1993; 1168(2):205-12. DOI: 10.1016/0005-2760(93)90126-t. View

del Prado M, Ramos R, Villalpando S . Lipid content and lipoprotein lipase activity in skeletal muscle of lactating and weaned rats. J Lipid Res. 1993; 34(7):1115-20. View

Da Costa T, Williamson D . Regulation of rat mammary-gland uptake of orally administered [1-14C]triolein by insulin and prolactin: evidence for bihormonal control of lipoprotein lipase activity. Biochem J. 1994; 300 ( Pt 1):257-62. PMC: 1138150. DOI: 10.1042/bj3000257. View

10.

Williamson D, Mellanby J, KREBS H . Enzymic determination of D(-)-beta-hydroxybutyric acid and acetoacetic acid in blood. Biochem J. 1962; 82:90-6. PMC: 1243411. DOI: 10.1042/bj0820090. View

11.

EGGSTEIN M, KREUTZ F . [A new determination of the neutral fats in blood serum and tissue. I. Principles, procedure, and discussion of the method]. Klin Wochenschr. 1966; 44(5):262-7. DOI: 10.1007/BF01747716. View

12.

HAMOSH M, Clary T, CHERNICK S, SCOW R . Lipoprotein lipase activity of adipose and mammary tissue and plasma triglyceride in pregnant and lactating rats. Biochim Biophys Acta. 1970; 210(3):473-82. DOI: 10.1016/0005-2760(70)90044-5. View

13.

Diamant S, GORIN E, Shafrir E . Enzyme activities related to fatty-acid synthesis in liver and adipose tissue of rats treated with triiodothyronine. Eur J Biochem. 1972; 26(4):553-9. DOI: 10.1111/j.1432-1033.1972.tb01798.x. View

14.

Lorscheider F, Mears G . Effects of extended lactation and 9 alpha-fluoroprednisolone acetate on serum thyroxine and lactational performance. J Endocrinol. 1973; 58(3):393-403. DOI: 10.1677/joe.0.0580393. View

15.

Mittra I . Mammotropic effect of prolactin enhanced by thyroidectomy. Nature. 1974; 248(448):525-6. DOI: 10.1038/248525a0. View

16.

Lu M, Anderson R . Thyroxine-induced hyperthyroidism upon the reproductive performance of female rats. Horm Res. 1975; 6(2):85-94. DOI: 10.1159/000178666. View

17.

Zinder O, Mendelson C, SCOW R . Lipoprotein lipase and uptake of chylomicron triacylglycerol and cholesterol by perfused rat mammary tissue. Biochim Biophys Acta. 1976; 431(3):526-37. DOI: 10.1016/0005-2760(76)90218-6. View

18.

Nilsson-Ehle P, Schotz M . A stable, radioactive substrate emulsion for assay of lipoprotein lipase. J Lipid Res. 1976; 17(5):536-41. View

19.

Linder C, CHERNICK S, Fleck T, SCOW R . Lipoprotein lipase and uptake of chylomicron triglyceride by skeletal muscle of rats. Am J Physiol. 1976; 231(3):860-4. DOI: 10.1152/ajplegacy.1976.231.3.860. View

20.

Stansbie D, Brownsey R, Crettaz M, Denton R . Acute effects in vivo of anti-insulin serum on rates of fatty acid synthesis and activities of acetyl-coenzyme A carboxylase and pyruvate dehydrogenase in liver and epididymal adipose tissue of fed rats. Biochem J. 1976; 160(2):413-6. PMC: 1164250. DOI: 10.1042/bj1600413. View