C-Myc Binds to 5' Flanking Sequence Motifs of the Dihydrofolate Reductase Gene in Cellular Extracts: Role in Proliferation

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1994 Jun 25

PMID 8036154

Citations 12

Authors

S Mai

A Jalava

Affiliations

Soon will be listed here.

Abstract

The dihydrofolate reductase is a key enzyme of the folate metabolism which supplies the cell with dTTPs for DNA synthesis. Using cellular extracts, we demonstrate the formation of c-Myc/Max heterodimers at the dihydrofolate reductase (DHFR) 5' flanking CANNTG (E-box) motifs. The presence of these complexes correlates with c-Myc levels and active cellular proliferation.

Citing Articles

LncEGFL7OS regulates human angiogenesis by interacting with MAX at the EGFL7/miR-126 locus.

Zhou Q, Yu B, Anderson C, Huang Z, Hanus J, Zhang W Elife. 2019; 8.

PMID: 30741632 PMC: 6370342. DOI: 10.7554/eLife.40470.

The Nutrient-Sensing Hexosamine Biosynthetic Pathway as the Hub of Cancer Metabolic Rewiring.

Chiaradonna F, Ricciardiello F, Palorini R Cells. 2018; 7(6).

PMID: 29865240 PMC: 6025041. DOI: 10.3390/cells7060053.

Gamabufotalin triggers c-Myc degradation via induction of WWP2 in multiple myeloma cells.

Yu Z, Li T, Wang C, Deng S, Zhang B, Huo X Oncotarget. 2016; 7(13):15725-37.

PMID: 26894970 PMC: 4941272. DOI: 10.18632/oncotarget.7398.

Transcriptional Regulation of CRD-BP by c-myc: Implications for c-myc Functions.

Noubissi F, Nikiforov M, Colburn N, Spiegelman V Genes Cancer. 2011; 1(10):1074-82.

PMID: 21779431 PMC: 3092266. DOI: 10.1177/1947601910395581.

The transcriptional program of a human B cell line in response to Myc.

Schuhmacher M, Kohlhuber F, Holzel M, Kaiser C, Burtscher H, Jarsch M Nucleic Acids Res. 2001; 29(2):397-406.

PMID: 11139609 PMC: 29676. DOI: 10.1093/nar/29.2.397.

References

Biedler J, Helson L, Spengler B . Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res. 1973; 33(11):2643-52. View

Berberich S, Espenshade P, Cole M . max encodes a sequence-specific DNA-binding protein and is not regulated by serum growth factors. Oncogene. 1992; 7(4):775-9. View

Biedler J, Schachner M, Freedman L . Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 1978; 38(11 Pt 1):3751-7. View

Shi Y, Glynn J, Guilbert L, Cotter T, Bissonnette R, Green D . Role for c-myc in activation-induced apoptotic cell death in T cell hybridomas. Science. 1992; 257(5067):212-4. DOI: 10.1126/science.1378649. View

Blackwood E, Kretzner L, Eisenman R . Myc and Max function as a nucleoprotein complex. Curr Opin Genet Dev. 1992; 2(2):227-35. DOI: 10.1016/s0959-437x(05)80278-3. View

Marcu K, Bossone S, Patel A . myc function and regulation. Annu Rev Biochem. 1992; 61:809-60. DOI: 10.1146/annurev.bi.61.070192.004113. View

Torres R, Schreiber-Agus N, Morgenbesser S, DePinho R . Myc and Max: a putative transcriptional complex in search of a cellular target. Curr Opin Cell Biol. 1992; 4(3):468-74. DOI: 10.1016/0955-0674(92)90013-3. View

Chisholm O, Stapleton P, Symonds G . Constitutive expression of exogenous myc in myelomonocytic cells: acquisition of a more transformed phenotype and inhibition of differentiation induction. Oncogene. 1992; 7(9):1827-36. View

Amati B, Dalton S, Brooks M, Littlewood T, Evan G, Land H . Transcriptional activation by the human c-Myc oncoprotein in yeast requires interaction with Max. Nature. 1992; 359(6394):423-6. DOI: 10.1038/359423a0. View

10.

Paria B, Dey S, Andrews G . Antisense c-myc effects on preimplantation mouse embryo development. Proc Natl Acad Sci U S A. 1992; 89(21):10051-5. PMC: 50275. DOI: 10.1073/pnas.89.21.10051. View

11.

Amin C, Wagner A, Hay N . Sequence-specific transcriptional activation by Myc and repression by Max. Mol Cell Biol. 1993; 13(1):383-90. PMC: 358918. DOI: 10.1128/mcb.13.1.383-390.1993. View

12.

Ayer D, Kretzner L, Eisenman R . Mad: a heterodimeric partner for Max that antagonizes Myc transcriptional activity. Cell. 1993; 72(2):211-22. DOI: 10.1016/0092-8674(93)90661-9. View

13.

Zervos A, Gyuris J, Brent R . Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites. Cell. 1993; 72(2):223-32. DOI: 10.1016/0092-8674(93)90662-a. View

14.

Fisher D, Parent L, Sharp P . High affinity DNA-binding Myc analogs: recognition by an alpha helix. Cell. 1993; 72(3):467-76. DOI: 10.1016/0092-8674(93)90122-7. View

15.

Evan G, Littlewood T . The role of c-myc in cell growth. Curr Opin Genet Dev. 1993; 3(1):44-9. DOI: 10.1016/s0959-437x(05)80339-9. View

16.

Cogliati T, Dunn B, Cultraro C, Segal S . Transfected wild-type and mutant max regulate cell growth and differentiation of murine erythroleukemia cells. Oncogene. 1993; 8(5):1263-8. View

17.

Jalava A, Akerman K, Heikkila J . Protein kinase inhibitor, staurosporine, induces a mature neuronal phenotype in SH-SY5Y human neuroblastoma cells through an alpha-, beta-, and zeta-protein kinase C-independent pathway. J Cell Physiol. 1993; 155(2):301-12. DOI: 10.1002/jcp.1041550211. View

18.

Blackwell T, Huang J, Ma A, Kretzner L, Alt F, Eisenman R . Binding of myc proteins to canonical and noncanonical DNA sequences. Mol Cell Biol. 1993; 13(9):5216-24. PMC: 360210. DOI: 10.1128/mcb.13.9.5216-5224.1993. View

19.

Packham G, Cleveland J . The ornithine decarboxylase gene is a transcriptional target of c-Myc. Proc Natl Acad Sci U S A. 1993; 90(16):7804-8. PMC: 47231. DOI: 10.1073/pnas.90.16.7804. View

20.

Roy A, Carruthers C, Gutjahr T, Roeder R . Direct role for Myc in transcription initiation mediated by interactions with TFII-I. Nature. 1993; 365(6444):359-61. DOI: 10.1038/365359a0. View