A Highly Sensitive System for the in Vitro Assembly of Bacteriophage Phi 29 of Bacillus Subtilis

Overview

Journal Virology

Specialty Microbiology

Date 1994 Aug 1

PMID 8030206

Citations 26

Authors

C S Lee

P Guo

Affiliations

Soon will be listed here.

Abstract

A sensitive system for the assay of bacteriophage phi 29 assembly in vitro was developed using 12 recombinant proteins and synthetic pRNA. This system detected in vitro assembled infectious phages up to 10(7) plaque forming units (PFU) per milliliter without any background. phi 29 DNA-gp3 concentration dependence in phage assembly was found to be first order, while the DNA-packaging protein gp16 dependence was higher order. The requirement for specific phi 29 pRNA for phi 29 DNA packaging was confirmed by the finding that no plaques were formed when only Escherichia coli RNAs were present. The activity of a mutant pRNA, with 10(5)-fold reduction in DNA packaging efficiency, was also demonstrated. Additionally, the tail proteins were found to have dual roles, one acting as phage tails and the other stabilizing the DNA-gp3 filled capsids.

Citing Articles

Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single-Directional Motion by Sequential Coordination.

Zhao Z, Zhang H, Shu D, Montemagno C, Ding B, Li J Small. 2016; 13(1).

PMID: 27709780 PMC: 5217803. DOI: 10.1002/smll.201601600.

An Arginine Finger Regulates the Sequential Action of Asymmetrical Hexameric ATPase in the Double-Stranded DNA Translocation Motor.

Zhao Z, De-Donatis G, Schwartz C, Fang H, Li J, Guo P Mol Cell Biol. 2016; 36(19):2514-23.

PMID: 27457616 PMC: 5021374. DOI: 10.1128/MCB.00142-16.

Development of Potent Antiviral Drugs Inspired by Viral Hexameric DNA-Packaging Motors with Revolving Mechanism.

Pi F, Zhao Z, Chelikani V, Yoder K, Kvaratskhelia M, Guo P J Virol. 2016; 90(18):8036-46.

PMID: 27356896 PMC: 5008075. DOI: 10.1128/JVI.00508-16.

Discovery of a new method for potent drug development using power function of stoichiometry of homomeric biocomplexes or biological nanomotors.

Pi F, Vieweger M, Zhao Z, Wang S, Guo P Expert Opin Drug Deliv. 2015; 13(1):23-36.

PMID: 26307193 PMC: 4713257. DOI: 10.1517/17425247.2015.1082544.

New approach to develop ultra-high inhibitory drug using the power function of the stoichiometry of the targeted nanomachine or biocomplex.

Shu D, Pi F, Wang C, Zhang P, Guo P Nanomedicine (Lond). 2015; 10(12):1881-97.

PMID: 26139124 PMC: 4733561. DOI: 10.2217/nnm.15.37.