Identification of a Gene Encoding a Thioredoxin-like Product Necessary for Cytochrome C Biosynthesis and Symbiotic Nitrogen Fixation in Rhizobium Leguminosarum

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1994 Jul 1

PMID 8021193

Citations 9

Authors

C Vargas

G Wu

A E Davies

J A Downie

Affiliations

Soon will be listed here.

Abstract

A Tn5-induced mutant of Rhizobium leguminosarum bv. viciae could not form nitrogen-fixing nodules on pea or vetch because of a lesion in electron transport to oxygen. The mutant lacked spectroscopically detectable cytochromes c and aa3. No proteins containing c-type cytochrome could be identified in the mutant by heme staining of proteins fractionated on polyacrylamide gels, indicating that the mutant was defective in maturation of all c-type cytochromes. The Tn5 mutation was determined to be located in a gene that was called cycY. The cycY gene product is homologous to the thioredoxin-like protein HelX involved in the assembly of c-type cytochromes in Rhodobacter capsulatus and to an open reading frame from a Bradyrhizobium japonicum gene cluster containing other genes involved in cytochrome c biogenesis. Our observations are consistent with CycY functioning as a thioredoxin that reduces cysteine residues in apocytochromes c before heme attachment.

Citing Articles

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Thiol-based redox signaling in the nitrogen-fixing symbiosis.

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