Endomysium Antibodies in Coeliac Disease: an Improved Method

Overview

Journal Gut

Specialty Gastroenterology

Date 1994 Jun 1

PMID 8020804

Citations 61

Authors

B Ladinser

E Rossipal

K Pittschieler

Affiliations

Soon will be listed here.

Abstract

The ultra structural binding sites of endomysium antibodies have been studied on human umbilical cord tissue. The sensitivity and specificity of IgA endomysium antibodies were compared with recently described methods using basement membrane of smooth muscle of monkey oesophagus. Thirty adults affected by coeliac disease (10 in remission) and 75 healthy adult controls with normal intestinal mucosa (35 false antigliadin positive) were investigated. Sensitivity and correlation of endomysium antibodies with total villous atrophy in untreated coeliac disease patients were 100% on the human umbilical cord smooth muscles, and only 90% on the muscular layer of primate oesophagus. Indirect immunofluorescence was superior to peroxidase staining in detecting these IgA antibodies. The easy availability and enhanced testing sensitivity of the umbilical cord is an advance towards a better diagnostic tool for coeliac disease.

Citing Articles

Association of concomitant autoimmunity with the disease features and long-term treatment and health outcomes in Celiac disease.

Tauschi R, Euren A, Vuorela N, Koskimaa S, Huhtala H, Kaukinen K Front Med (Lausanne). 2022; 9:1055135.

PMID: 36465913 PMC: 9709120. DOI: 10.3389/fmed.2022.1055135.

Significance of low ferritin without anaemia in screen-detected, adult coeliac disease patients.

Repo M, Kurppa K, Huhtala H, Luostarinen L, Kaukinen K, Kivela L J Intern Med. 2022; 292(6):904-914.

PMID: 35925673 PMC: 9805163. DOI: 10.1111/joim.13548.

Type 2 Transglutaminase in Coeliac Disease: A Key Player in Pathogenesis, Diagnosis and Therapy.

Paolella G, Sposito S, Romanelli A, Caputo I Int J Mol Sci. 2022; 23(14).

PMID: 35886862 PMC: 9318967. DOI: 10.3390/ijms23147513.

Persistent symptoms are diverse and associated with health concerns and impaired quality of life in patients with paediatric coeliac disease diagnosis after transition to adulthood.

Vuolle S, Laurikka P, Repo M, Huhtala H, Kaukinen K, Kurppa K BMJ Open Gastroenterol. 2022; 9(1).

PMID: 35820709 PMC: 9277401. DOI: 10.1136/bmjgast-2022-000914.

A No-Biopsy Approach for the Diagnosis of Celiac Disease in Adults: Can It Be Real?.

Baykan A, Cerrah S, Ciftel S, Vural M, Kasap E Cureus. 2022; 14(7):e26521.

PMID: 35795577 PMC: 9250690. DOI: 10.7759/cureus.26521.

References

Chorzelski T, BEUTNER E, Sulej J, Tchorzewska H, Jablonska S, Kumar V . IgA anti-endomysium antibody. A new immunological marker of dermatitis herpetiformis and coeliac disease. Br J Dermatol. 1984; 111(4):395-402. DOI: 10.1111/j.1365-2133.1984.tb06601.x. View

. Revised criteria for diagnosis of coeliac disease. Report of Working Group of European Society of Paediatric Gastroenterology and Nutrition. Arch Dis Child. 1990; 65(8):909-11. PMC: 1792502. DOI: 10.1136/adc.65.8.909. View

Mantzaris G, Rosenberg W, Jewell D . The immunology of coeliac disease. Springer Semin Immunopathol. 1990; 12(2-3):219-29. DOI: 10.1007/BF00197507. View

Valeski J, Kumar V, BEUTNER E, Lerner A, Chorzelski T . Immunology of celiac disease: tissue and species specificity of endomysial and reticulin antibodies. Int Arch Allergy Appl Immunol. 1990; 93(1):1-7. DOI: 10.1159/000235271. View

Kelly C, Feighery C, Gallagher R, Gibney M, Weir D . Mucosal and systemic IgA anti-gliadin antibody in celiac disease. Contrasting patterns of response in serum, saliva, and intestinal secretions. Dig Dis Sci. 1991; 36(6):743-51. DOI: 10.1007/BF01311231. View

HEKKENS W . Coeliac disease: digestive, immunologic, and nutritional implications. Bibl Nutr Dieta. 1992; (50):58-71. DOI: 10.1159/000421889. View

Troncone R, Ferguson A . Anti-gliadin antibodies. J Pediatr Gastroenterol Nutr. 1991; 12(2):150-8. DOI: 10.1097/00005176-199102000-00002. View

Marsh M . Gluten, major histocompatibility complex, and the small intestine. A molecular and immunobiologic approach to the spectrum of gluten sensitivity ('celiac sprue'). Gastroenterology. 1992; 102(1):330-54. View

Karpati S, Meurer M, Stolz W, Burgin-Wolff A, Braun-Falco O, Krieg T . Ultrastructural binding sites of endomysium antibodies from sera of patients with dermatitis herpetiformis and coeliac disease. Gut. 1992; 33(2):191-3. PMC: 1373928. DOI: 10.1136/gut.33.2.191. View

10.

Ferreira M, Davies S, Butler M, Scott D, Clark M, Kumar P . Endomysial antibody: is it the best screening test for coeliac disease?. Gut. 1992; 33(12):1633-7. PMC: 1379574. DOI: 10.1136/gut.33.12.1633. View

11.

Volta U, Molinaro N, Fusconi M, Cassani F, Bianchi F . IgA antiendomysial antibody test. A step forward in celiac disease screening. Dig Dis Sci. 1991; 36(6):752-6. DOI: 10.1007/BF01311232. View