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Measurement of Four-bond HN-H Alpha J-couplings in Staphylococcal Nuclease

Overview
Journal J Biomol NMR
Publisher Springer
Specialties Molecular Biology
Nuclear Medicine
Date 1994 Mar 1
PMID 8019134
Citations 16
Authors
G W Vuister
A Bax
Affiliations
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Abstract

Quantitative J-correlation and triple-resonance ECOSY-type experiments are used to unambiguously establish the presence of four-bond sequential HN-H alpha J-couplings in the protein staphylococcal nuclease. Substantially negative 4JH alpha HN values, ranging from -0.8 to -2.3 Hz, are observed when the psi angle is near +120 degrees, and the following phi angle near +60 degrees. For other conformations, the four-bond HN-H alpha J-couplings fall between -0.5 and +0.5 Hz.

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References
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