Characterization of an Avirulent Mutant of a Virulent Avian Escherichia Coli Isolate

Overview

Journal Avian Dis

Specialty Veterinary Medicine

Date 1994 Jan 1

PMID 8002883

Citations 4

Authors

L K Nolan

R E Wooley

C W Giddings

J Brown

Affiliations

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Abstract

A virulent, complement-resistant avian Escherichia coli isolate and its avirulent, complement-sensitive, transposon-insertion mutant were compared for the purpose of revealing structures associated with complement resistance. Both had a smooth lipopolysaccharide layer, contained traT, and lacked a capsule, but the mutant possessed a 16.2-kilodalton outer-membrane protein (OMP) not present in the wild-type. This protein may be the product of a coding region interrupted by transposon insertion. Such results suggest that an OMP greater than 16.2 kilodaltons in size may be responsible for the complement resistance and virulence of this wild-type E. coli.

Citing Articles

Characterization of Escherichia coli isolates incriminated in colisepticaemia in mink.

Tibbetts R, White D, Dyer N, Giddings C, Nolan L Vet Res Commun. 2003; 27(5):341-57.

PMID: 14509449 DOI: 10.1023/a:1024741719361.

Resistance to serum complement, iss, and virulence of avian Escherichia coli.

Nolan L, Horne S, Giddings C, Foley S, Johnson T, Lynne A Vet Res Commun. 2003; 27(2):101-10.

PMID: 12718504 DOI: 10.1023/a:1022854902700.

Role of virulence factors in resistance of avian pathogenic Escherichia coli to serum and in pathogenicity.

Mellata M, Dho-Moulin M, Dozois C, Curtiss 3rd R, Brown P, Arne P Infect Immun. 2002; 71(1):536-40.

PMID: 12496207 PMC: 143143. DOI: 10.1128/IAI.71.1.536-540.2003.

Characterization of Salmonella dublin and Salmonella typhimurium (Copenhagen) isolates from cattle.

Brackelsberg C, Nolan L, Brown J Vet Res Commun. 1997; 21(6):409-20.

PMID: 9266660 DOI: 10.1023/a:1005803301827.