Article: A densovirus newly isolated from the smoky-brown cockroach Periplaneta fuliginosa

We purified a causing agent of fetal disease for smoky-brown cockroach Periplaneta fuliginosa, which was designated as "cockroach small spherical virus (CSSV)". Purified virus particles had a diameter of 22 +/- 0.6 nm and contained DNA as a single-stranded form. However, the extraction of DNA under condition of appropriate high salt and elevated temperature yielded a double-stranded DNA with a size of 5,500 nucleotides. These results were quite similar to those of other densoviruses (DNVs). The CSSV had five structural proteins (VP1: 52 KDa, VP2: 56 KDa, VP3: 79 KDa, VP4: 82 KDa, and VP5: 105 KDa). The SDS-PAGE profile of these proteins was quite different from that of the cockroach DNV previously reported and was rather similar to that of Bombyx mori (Bm) DNV-1. An immunochemical study, however, demonstrated that there was no immunological relationship between the CSSV and the Bm DNV-1. These data suggest that the CSSV is a new member of DNV.

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References

1.

Astell C, Thomson M, Merchlinsky M, Ward D . The complete DNA sequence of minute virus of mice, an autonomous parvovirus. Nucleic Acids Res. 1983; 11(4):999-1018. PMC: 325773. DOI: 10.1093/nar/11.4.999. View

2.

LERMAN L . The structure of the DNA-acridine complex. Proc Natl Acad Sci U S A. 1963; 49:94-102. PMC: 300634. DOI: 10.1073/pnas.49.1.94. View

3.

Bando H, Kondo N, Kawase S . Molecular homology among the structural proteins of densonucleosis virus from silkworm, Bombyx mori. Arch Virol. 1984; 80(2-3):209-18. DOI: 10.1007/BF01310660. View

4.

Blake A, PEACOCKE A . The interaction of aminocridines with nucleic acids. Biopolymers. 1968; 6(9):1225-53. DOI: 10.1002/bip.1968.360060902. View

5.

Kelly D, Barwise A, Walker I . DNA contained by two densonucleosis viruses. J Virol. 1977; 21(1):396-407. PMC: 353826. DOI: 10.1128/JVI.21.1.396-407.1977. View

6.

Bando H, Choi H, Ito Y, Kawase S . Terminal structure of a Densovirus implies a hairpin transfer replication which is similar to the model for AAV. Virology. 1990; 179(1):57-63. DOI: 10.1016/0042-6822(90)90273-t. View

7.

Tattersall P, Ward D . Rolling hairpin model for replication of parvovirus and linear chromosomal DNA. Nature. 1976; 263(5573):106-9. DOI: 10.1038/263106a0. View

8.

Bando H, Choi H, Ito Y, Nakagaki M, Kawase S . Structural analysis on the single-stranded genomic DNAs of the virus newly isolated from silkworm: the DNA molecules share a common terminal sequence. Arch Virol. 1992; 124(1-2):187-93. DOI: 10.1007/BF01314637. View

9.

Suto C . Characterization of a virus newly isolated from the smoky-brown cockroach, Periplaneta fuliginosa (Serville). Nagoya J Med Sci. 1979; 42(1-2):13-25. View

10.

Kurstak E . Small DNA densonucleosis virus (DNV). Adv Virus Res. 1972; 17:207-41. DOI: 10.1016/s0065-3527(08)60751-4. View

11.

Berns K, Kelly Jr T . Letter: Visualization of the inverted terminal repetition in adeno-associated virus DNA. J Mol Biol. 1974; 82(2):267-71. DOI: 10.1016/0022-2836(74)90344-1. View

A Densovirus Newly Isolated from the Smoky-brown Cockroach Periplaneta Fuliginosa