PCR-based Immortalization and Screening of Hierarchical Pools of CDNAs

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1994 Nov 11

PMID 7984433

Citations 1

Authors

M DEsposito

R Mazzarella

G Pengue

C Jones

M DUrso

D Schlessinger

Affiliations

Soon will be listed here.

Abstract

Starting from sequences of at least 60 bp, PCR-based screening has been developed to recover cDNAs from libraries without the necessity for hybridization or extensive DNA extraction steps. The method maintains the indefinite availability of even scarce cDNA libraries and provides an estimate of the relative abundance of the mRNA species. Isolation of a cDNA clone can be done in less than a week. cDNAs were isolated that were cognate for fragments of expressed sequences and for an exon predicted from genomic sequence.

Citing Articles

RAPD-based screening of genomic libraries for positional cloning.

Dioh W, Tharreau D, Lebrun M Nucleic Acids Res. 1998; 25(24):5130-1.

PMID: 9396827 PMC: 147131. DOI: 10.1093/nar/25.24.5130.

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