Nucleotide Sequences and Expression in Escherichia Coli of the Coat Protein Genes from Two Strains of Melon Necrotic Spot Virus

Overview

Journal Arch Virol

Specialty Microbiology

Date 1994 Jan 1

PMID 7980005

Authors

K Ohshima

K Matsuo

N Sako

Affiliations

Soon will be listed here.

Abstract

The nucleotide sequences of coat protein (CP) genes of two Japanese strains of melon necrotic spot virus (MNSV-NH and MNSV-S) were determined. The size of the CP genes of both strains was 1170 nucleotides. Comparisons of the deduced amino acid sequences among MNSV strains showed more than 95% homology, and those among other carmoviruses showed 31-34% homology. cDNAs of MNSV CP genes were cloned into an Escherichia coli expression fusion vector and MNSV-NH and MNSV-S CPs were successfully produced. Furthermore, synthetic oligonucleotide primers were used for differentiating MNSV strains by reverse transcription-polymerase chain reaction method.

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