Uptake and Phototoxic Effects of Aluminum-chlorophthalocyanine (AlSPc) in Human Bladder Carcinoma Cells

Overview

Journal Urol Res

Specialty Urology

Date 1994 Jan 1

PMID 7974917

Authors

K Miller

E Reich

T Grau

R Hautmann

Affiliations

Soon will be listed here.

Abstract

In vitro experiments were performed on human bladder carcinoma cells to evaluate the uptake of aluminum-chlorophthalocyanine (AISPc) and the subcellular target of phototoxicity. In order to quantify the correlation of intracellular uptake and incubation time and to identify the primary subcellular target of phototoxicity, fluorescence and absorption measurements have been carried out as well as electron microscopic studies. Absorption and fluorescence measurements showed the largest value after 24 h of incubation time. Fluorescence microscopic studies suggested the sensitizer to be located in a brighter patch within cytoplasm. Electron microscopic studies using DAB (3,3' diaminobenzidine) staining showed that the mitochondria are the primary target of phototoxic activity of AlSPc and that the majority of vacuoles of treated cells were originally mitochondria.

References

Berns M, Dahlman A, Johnson F, Burns R, Sperling D, Guiltinan M . In vitro cellular effects of hematoporphyrin derivative. Cancer Res. 1982; 42(6):2325-9. View

Chan W, Marshall J, Hart I . Effect of tumour location on selective uptake and retention of phthalocyanines. Cancer Lett. 1989; 44(1):73-7. DOI: 10.1016/0304-3835(89)90111-0. View

Ben-Hur E, Green M, Prager A, Kol R, Rosenthal I . Phthalocyanine photosensitization of mammalian cells: biochemical and ultrastructural effects. Photochem Photobiol. 1987; 46(5):651-6. DOI: 10.1111/j.1751-1097.1987.tb04827.x. View

Ben-Hur E, Rosenthal I . Photosensitized inactivation of Chinese hamster cells by phthalocyanines. Photochem Photobiol. 1985; 42(2):129-33. DOI: 10.1111/j.1751-1097.1985.tb01550.x. View

Shulok J, Klaunig J, Selman S, Schafer P, Goldblatt P . Cellular effects of hematoporphyrin derivative photodynamic therapy on normal and neoplastic rat bladder cells. Am J Pathol. 1986; 122(2):277-83. PMC: 1888108. View

Schneckenburger H, Ruck A, Bartos B, Steiner R . Intracellular distribution of photosensitizing porphyrins measured by video-enhanced fluorescence microscopy. J Photochem Photobiol B. 1988; 2(3):355-63. DOI: 10.1016/1011-1344(88)85054-1. View

Komatsu K . Photodynamic cell killing effects and acute skin photosensitivity of aluminum-chloro-tetrasulfonated phthalocyanine and hematoporphyrin derivative. Jpn J Cancer Res. 1991; 82(5):599-606. PMC: 5918468. DOI: 10.1111/j.1349-7006.1991.tb01892.x. View

Dixit R, Mukhtar H, Bickers D . Destruction of microsomal cytochrome P-450 by reactive oxygen species generated during photosensitization of hematoporphyrin derivative. Photochem Photobiol. 1983; 37(2):173-6. DOI: 10.1111/j.1751-1097.1983.tb04454.x. View

Berg K, Bommer J, Moan J . Evaluation of sulfonated aluminum phthalocyanines for use in photochemotherapy. A study on the relative efficiencies of photoinactivation. Photochem Photobiol. 1989; 49(5):587-94. DOI: 10.1111/j.1751-1097.1989.tb08428.x. View

10.

HILF R, Murant R, Narayanan U, Gibson S . Relationship of mitochondrial function and cellular adenosine triphosphate levels to hematoporphyrin derivative-induced photosensitization in R3230AC mammary tumors. Cancer Res. 1986; 46(1):211-7. View

11.

Miller K, Reich E, Ruck A, Hautmann R . Photodynamic therapy of bladder cancer--uptake and phototoxicity of photosan in vitro. Urol Res. 1991; 19(6):353-6. DOI: 10.1007/BF00310149. View

12.

Shea C, Wimberly J, Hasan T . Mitochondrial phototoxicity sensitized by doxycycline in cultured human carcinoma cells. J Invest Dermatol. 1986; 87(3):338-42. DOI: 10.1111/1523-1747.ep12524430. View

13.

Henderson B, Dougherty T . How does photodynamic therapy work?. Photochem Photobiol. 1992; 55(1):145-57. DOI: 10.1111/j.1751-1097.1992.tb04222.x. View

14.

Wagner J, Ali H, Langlois R, Brasseur N, van Lier J . Biological activities of phthalocyanines--VI. Photooxidation of L-tryptophan by selectively sulfonated gallium phthalocyanines: singlet oxygen yields and effect of aggregation. Photochem Photobiol. 1987; 45(5):587-94. DOI: 10.1111/j.1751-1097.1987.tb07384.x. View

15.

Paquette B, Ali H, Langlois R, van Lier J . Biological activities of phthalocyanines--VIII. Cellular distribution in V-79 Chinese hamster cells and phototoxicity of selectively sulfonated aluminum phthalocyanines. Photochem Photobiol. 1988; 47(2):215-20. DOI: 10.1111/j.1751-1097.1988.tb02717.x. View