Polarity and Gradients in Lepidopteran Wing Epidermis. I. Changes in Graft Polarity, Form, and Cell Density Accompanying Transpositions and Reorientations

Lepidopteran wing epidermis has certain advantages for studying the spatial organization of cell populations: ease of accessibility and manipulation, large size, an essentially two-dimensional structure, and direct expression by the scale cells of their polarity. Grafting experiments reveal that polarity and density of graft cells as well as overall graft form are functions of a graft's source and its transplantation site; graft polarity is also determined by the orientation of the graft. The results are consistent with the existence of at least one morphogenetic gradient along the proximo-distal axis of the wing's upper epidermal layer. Various gradient models that might explain the experimental observations are considered.