Increased Endothelin-1 Production in Fibroblasts Derived from Patients with Systemic Sclerosis

Overview

Journal Ann Rheum Dis

Specialty Rheumatology

Date 1994 Aug 1

PMID 7944634

Citations 22

Authors

Y Kawaguchi

K Suzuki

M Hara

T Hidaka

T Ishizuka

M Kawagoe

H Nakamura

Affiliations

Soon will be listed here.

Abstract

Objectives: To determine whether fibroblasts from patients with systemic sclerosis (SSc) produce excessive amounts of endothelin-1 (ET-1), which is recognised as having vasoconstrictive properties and as having a potent mitogenic effect on fibroblasts.

Methods: Dermal fibroblasts were removed from 11 patients with SSc and from five normal controls (NC). The assay of ET-1 protein was measured by an ELISA that used two anti-ET-1 antibodies. The gene expression of prepro ET-1 mRNA was evaluated by a reverse-transcriptase polymerase chain reaction (RT-PCR) method.

Results: Levels of ET-1 protein were significantly higher in SSc fibroblast cultures than in those of normal fibroblasts (p < 0.01). The expression of prepro ET-1 mRNA was also higher in SSc fibroblasts than in normal fibroblasts. The addition of interleukin-1 beta (IL-1 beta) increased the production of ET-1 by fibroblasts.

Conclusion: The findings indicate that the overproduction of ET-1 is a novel abnormal function in SSc fibroblasts, and that ET-1 induced by fibroblasts may play a role in the fibrosis and Raynaud's phenomenon of SSc.

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