Alpha 2.0
Login Register
» Articles » PMID: 7926885

High-resolution 1H NMR Spectroscopy of Aqueous Humour from Rabbits

Overview
Journal Graefes Arch Clin Exp Ophthalmol
Specialty Ophthalmology
Date 1994 Aug 1
PMID 7926885
Citations 2
Authors
I S Gribbestad
A Midelfart
Affiliations
Soon will be listed here.
Abstract

Background: Detection of different substances in aqueous humour is important for evaluation of the disorders affecting the eye. The purpose of the present study was to apply high-resolution proton (1H) nuclear magnetic resonance (NMR) spectroscopy for extensive characterisation of the metabolites in the aqueous humour from rabbits.

Methods: High-resolution 1H NMR spectroscopy, including two-dimensional shift correlated (COSY) technique, was performed on aqueous humour from rabbits.

Results: More than 20 metabolites were simultaneously detected and identified in high-resolution 1H NMR spectra of aqueous humour from rabbits. Some of these were also quantified.

Conclusion: High-resolution 1H NMR spectroscopy is a valuable method for simultaneous detection of many different metabolites in aqueous humour.

Citing Articles

Retinal metabolic changes in an experimental model of optic nerve transection by ex vivo 1H magnetic resonance spectroscopy.

Li S, Huang M, Wang X, Wang X, Chen F, Lei H Neurochem Res. 2011; 36(12):2427-33.

PMID: 21842271 DOI: 10.1007/s11064-011-0570-7.

Changes in aqueous humour following single or repeated UVB irradiation of rabbit cornea.

Fris M, cejkova J, Midelfart A Graefes Arch Clin Exp Ophthalmol. 2007; 245(11):1705-11.

PMID: 17605025 DOI: 10.1007/s00417-007-0620-7.

References
1.
Reddy D, Rosenberg C, KINSEY V . Steady state distribution of free amino acids in the aqueous humours, vitreous body and plasma of the rabbit. Exp Eye Res. 1961; 1:175-91. DOI: 10.1016/s0014-4835(61)80024-9. View
2.
Cerdan S, Parrilla R, Santoro J, Rico M . 1H NMR detection of cerebral myo-inositol. FEBS Lett. 1985; 187(1):167-72. DOI: 10.1016/0014-5793(85)81235-7. View
3.
Brown J, Sadler P, Spalton D, Juul S, Macleod A, Sonksen P . Analysis of human aqueous humour by high resolution 1H NMR spectroscopy. Exp Eye Res. 1986; 42(4):357-62. DOI: 10.1016/0014-4835(86)90029-1. View
4.
Behar K, Ogino T . Assignment of resonance in the 1H spectrum of rat brain by two-dimensional shift correlated and J-resolved NMR spectroscopy. Magn Reson Med. 1991; 17(2):285-303. DOI: 10.1002/mrm.1910170202. View
5.
Evanochko W, Sakai T, Ng T, Krishna N, Kim H, Zeidler R . NMR study of in vivo RIF-1 tumors. Analysis of perchloric acid extracts and identification of 1H, 31P and 13C resonances. Biochim Biophys Acta. 1984; 805(1):104-16. DOI: 10.1016/0167-4889(84)90042-9. View