Catabolite Translational Effects on the Lac Messenger RNA of Escherichia Coli K12

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1976 Oct 18

PMID 792685

Citations 1

Authors

C Sanchez de Rivas

B S Mendez

Affiliations

Soon will be listed here.

Abstract

The transcriptional and translational events occurring during the induction of the lac operon, were separated by blocking the translational step, either by aminoacid starvation or by addition of chloramphenicol. It was found that the carbon source used during the subsequent translation, affected the rate of beta-galactosidase synthesis. A decoordination effect on the production of enzymes of the lac system was also observed in high catabolite repression media, as well as in nitrogen limiting conditions. These findings suggested a similarity with the polarity phenomenon. In order to test this similarity, polarity suppressors of a Z- polar mutant were isolated. In one of these mutants, probably suA like, no carbon source effect was observed during the translational step. The induction kinetics in different media, after distinct pregrowth conditions, supported the idea that this mutant could be considered catabolite repression resistant only in certain restrictive conditions.

Citing Articles

Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation.

Sanchez de Rivas C, Mendez B Mol Gen Genet. 1977; 156(2):229-32.

PMID: 340904 DOI: 10.1007/BF00283496.

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