Human TAFII30 is Present in a Distinct TFIID Complex and is Required for Transcriptional Activation by the Estrogen Receptor

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 1994 Oct 7

PMID 7923369

Citations 129

Authors

X Jacq

C Brou

Y Lutz

I Davidson

P Chambon

L Tora

Affiliations

Soon will be listed here.

Abstract

We showed previously that coactivators mediating stimulation by different activators were associated with the TATA-binding protein (TBP) in distinct TFIID complexes. We have characterized a human TBP-associated factor (TAF), hTAFII30, associated with a subset of TFIID complexes. hTAFII30 interacts with the AF-2-containing region E of the human estrogen receptor (ER), but not with ER AF-1 or VP16. An antibody against hTAFII30 inhibited transcriptional stimulation by the ER AF-2 without affecting basal or VP16-activated transcription and allowed the separation of TFIID complex(es) containing hTAFII30 from complexes mediating the activity of VP16. These results directly demonstrate the existence of functionally distinct TFIID populations that share common TAFIIs but differ in specific TAFIIs.

Citing Articles

RNA polymerase II transcription initiation in holo-TFIID-depleted mouse embryonic stem cells.

Hisler V, Bardot P, Detilleux D, Bernardini A, Stierle M, Sanchez E Cell Rep. 2024; 43(10):114791.

PMID: 39352809 PMC: 11551524. DOI: 10.1016/j.celrep.2024.114791.

Fischer V, Plassard D, Ye T, Reina-San-Martin B, Stierle M, Tora L Cell Rep. 2021; 36(8):109598.

PMID: 34433046 PMC: 8430043. DOI: 10.1016/j.celrep.2021.109598.

Co-translational assembly of mammalian nuclear multisubunit complexes.

Kamenova I, Mukherjee P, Conic S, Mueller F, El-Saafin F, Bardot P Nat Commun. 2019; 10(1):1740.

PMID: 30988355 PMC: 6465333. DOI: 10.1038/s41467-019-09749-y.

The TAF10-containing TFIID and SAGA transcriptional complexes are dispensable for early somitogenesis in the mouse embryo.

Bardot P, Vincent S, Fournier M, Hubaud A, Joint M, Tora L Development. 2017; 144(20):3808-3818.

PMID: 28893950 PMC: 5650055. DOI: 10.1242/dev.146902.

Regulatory Enhancer-Core-Promoter Communication via Transcription Factors and Cofactors.

Zabidi M, Stark A Trends Genet. 2016; 32(12):801-814.

PMID: 27816209 PMC: 6795546. DOI: 10.1016/j.tig.2016.10.003.