Polyamine and Ornithine Metabolism During the Germination of Conidia of Aspergillus Nidulans

Overview

Journal Biochem J

Specialty Biochemistry

Date 1976 Aug 15

PMID 791270

Citations 4

Authors

L Stevens

I M McKinnon

M Winther

Affiliations

Soon will be listed here.

Abstract

1. The activities of ornithine decarboxylase, S-adenosylmethionine decarboxylase and ornithine-2-oxoglutarate aminotransferase were studied during the first 24 h of conidial germination in Aspergillus nidulans. 2. Increases (over 100-fold) in the activities of ornithine decarboxylase and S-adenosylmethionine decarboxylase occurred during the emergence of the germ-tube and before the doubling of DNA and this was followed by a sharp fall in the activities of both enzymes by 16h. 3. The increase in ornithine decarboxylase could be largely suppressed if 0.6 mM-putrescine was added to the growth medium. 4. Low concentrations of cycloheximide, which delayed germination by 2h, caused a corresponding delay in the changes in ornithine decarboxylase activity. 5. Ornithine-2-oxoglutarate aminotransferase activity increased steadily during the first 24h of germination. 6. Ornithine or arginine in the growth medium induced higher activity of ornithine-2-oxoglutarate aminotransferase, but did not affect ornithine decarboxylase activity. 7. The significance of these enzyme changes during germination is discussed.

Citing Articles

Osmotically induced changes in the ornithine decarboxylase activity of Dictyostelium discoideum.

Harris W, North M J Bacteriol. 1982; 150(2):716-21.

PMID: 7068532 PMC: 216421. DOI: 10.1128/jb.150.2.716-721.1982.

Polyamine biosynthesis during germination of yeast ascospores.

Brawley J, Ferro A J Bacteriol. 1979; 140(2):649-54.

PMID: 387744 PMC: 216693. DOI: 10.1128/jb.140.2.649-654.1979.

The effect of 1,4-diaminobutanone on the stability of ornithine decarboxylase from Aspergillus nidulans.

Stevens L, McKinnon I Biochem J. 1977; 166(3):635-7.

PMID: 339910 PMC: 1165050. DOI: 10.1042/bj1660635.

Some genetical aspects of ornithine metabolism in Aspergillus nidulans.

Arst Jr H Mol Gen Genet. 1977; 151(1):105-10.

PMID: 325361 DOI: 10.1007/BF00446919.

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