Relaxation of Replication Control in Chaperone-independent Initiator Mutants of Plasmid P1

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1994 May 1

PMID 7910558

Citations 10

Authors

G Mukhopadhyay

S Sozhamannan

D K Chattoraj

Affiliations

Soon will be listed here.

Abstract

Escherichia coli chaperones DnaJ, DnaK and GrpE increase P1 plasmid initiator binding to the origin by promoting initiator folding. The binding allows initiation and also promotes pairing of origins which is believed to control initiation frequency. Chaperone-independent DNA binding mutants are often defective in replication control. We show here that these mutants have increased rates of association for DNA binding and defects in origin pairing. The increases in association rates were found to be due either to increased protein folding into active forms or to increases in the association rate constant, kon. Since the dissociation rate constants for DNA release with these mutants are not changed, it is unlikely that the DNA binding domain is affected. The pairing domain may thus control replication and modulate DNA binding. The role of the pairing domain in DNA binding can be significant in vivo as the selection for chaperone-independent binding favors pairing-defective mutants.

Citing Articles

The DnaK Chaperone Uses Different Mechanisms To Promote and Inhibit Replication of Chromosome 2.

Jha J, Li M, Ghirlando R, Miller Jenkins L, Wlodawer A, Chattoraj D mBio. 2017; 8(2).

PMID: 28420739 PMC: 5395669. DOI: 10.1128/mBio.00427-17.

Role of pi dimers in coupling ("handcuffing") of plasmid R6K's gamma ori iterons.

Kunnimalaiyaan S, Inman R, Rakowski S, Filutowicz M J Bacteriol. 2005; 187(11):3779-85.

PMID: 15901701 PMC: 1112066. DOI: 10.1128/JB.187.11.3779-3785.2005.

Multiple homeostatic mechanisms in the control of P1 plasmid replication.

Das N, Valjavec-Gratian M, Basuray A, Fekete R, Papp P, Paulsson J Proc Natl Acad Sci U S A. 2005; 102(8):2856-61.

PMID: 15708977 PMC: 549481. DOI: 10.1073/pnas.0409790102.

Origin pairing ('handcuffing') as a mode of negative control of P1 plasmid copy number.

Park K, Han E, Paulsson J, Chattoraj D EMBO J. 2001; 20(24):7323-32.

PMID: 11743008 PMC: 125786. DOI: 10.1093/emboj/20.24.7323.

Molecular analysis of the Doppia transposable element of maize.

Bercury S, Panavas T, Irenze K, WALKER E Plant Mol Biol. 2001; 47(3):341-51.

PMID: 11587506 DOI: 10.1023/a:1011606529513.

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