Regulation of Ammonium Ion Assimilation Enzymes in Neurospora Crassa Nit-2 and Ms-5 Mutant Strains

Overview

Journal Biochem Genet

Specialty Molecular Biology

Date 1993 Oct 1

PMID 7907211

Citations 3

Authors

J Calderon

L M Martinez

Affiliations

Soon will be listed here.

Abstract

In Neurospora crassa the nit-2 and nmr-1 (ms-5) loci represent the major control genes encoding regulatory proteins that allow the coordinated expression of various systems involved with the utilization of a secondary nitrogen source. In this paper we examined the effect of the nit-2 and ms-5 (nmr-1 locus) mutations on the regulation of the ammonium assimilation enzymes, glutamine synthetase and glutamate dehydrogenase, which are regulated by the products of these genes; however, glutamate synthase is not so regulated. Glutamine synthetase and glutamate dehydrogenase levels are also regulated by the amino nitrogen content. We present evidence that the ms-5 and glnr strains, which behave very similarly in their resistance to glutamine repression, are different and map in different loci.

Citing Articles

A sensing role of the glutamine synthetase in the nitrogen regulation network in Fusarium fujikuroi.

Wagner D, Wiemann P, Huss K, Brandt U, Fleissner A, Tudzynski B PLoS One. 2013; 8(11):e80740.

PMID: 24260467 PMC: 3829961. DOI: 10.1371/journal.pone.0080740.

Identification of two glutaminases in Rhizobium etli.

Duran S, Huerta-Saquero A, Du Pont G, Huerta-Zepeda A, Calderon J Biochem Genet. 1996; 34(11-12):453-65.

PMID: 9126674 DOI: 10.1007/BF00570126.

Isolation and characterization of Rhizobium etli mutants altered in degradation of asparagine.

Huerta-Zepeda A, Ortuno L, Du Pont G, Duran S, Lloret A, Merchant-Larios H J Bacteriol. 1997; 179(6):2068-72.

PMID: 9068657 PMC: 178935. DOI: 10.1128/jb.179.6.2068-2072.1997.

References

Kinnaird J, Keighren M, Kinsey J, Eaton M, Fincham J . Cloning of the am (glutamate dehydrogenase) gene of Neurospora crassa through the use of a synthetic DNA probe. Gene. 1982; 20(3):387-96. DOI: 10.1016/0378-1119(82)90207-4. View

Lara M, Blanco L, Campomanes M, Calva E, Palacios R, Mora J . Physiology of ammonium assimilation in Neurospora crassa. J Bacteriol. 1982; 150(1):105-12. PMC: 220087. DOI: 10.1128/jb.150.1.105-112.1982. View

Marzluf G . Regulation of nitrogen metabolism and gene expression in fungi. Microbiol Rev. 1981; 45(3):437-61. PMC: 281519. DOI: 10.1128/mr.45.3.437-461.1981. View

Hummelt G, Mora J . NADH-dependent glutamate synthase and nitrogen metabolism in Neurospora crassa. Biochem Biophys Res Commun. 1980; 92(1):127-33. DOI: 10.1016/0006-291x(80)91529-6. View

Palacios R . Neurospora crassa glutamine synthetase. Purification by affinity chromatography and characterization of subunit structure. J Biol Chem. 1976; 251(15):4787-91. View

Fu Y, Young J, Marzluf G . Molecular cloning and characterization of a negative-acting nitrogen regulatory gene of Neurospora crassa. Mol Gen Genet. 1988; 214(1):74-9. DOI: 10.1007/BF00340182. View

Ronzio R, Meister A . Phosphorylation of methionine sulfoximine by glutamine synthetase. Proc Natl Acad Sci U S A. 1968; 59(1):164-70. PMC: 286017. DOI: 10.1073/pnas.59.1.164. View

Hummelt G, Mora J . Regulation and function of glutamate synthase in Neurospora crassa. Biochem Biophys Res Commun. 1980; 96(4):1688-94. DOI: 10.1016/0006-291x(80)91368-6. View

Cove D . Chlorate toxicity in Aspergillus nidulans. Studies of mutants altered in nitrate assimilation. Mol Gen Genet. 1976; 146(2):147-59. DOI: 10.1007/BF00268083. View

10.

Facklam T, Marzluf G . Nitrogen regulation of amino acid catabolism in Neurospora crassa. Biochem Genet. 1978; 16(3-4):343-54. DOI: 10.1007/BF00484090. View

11.

Seale T, Brett M, Baron A, Fincham J . Amino acid replacements resulting from suppression and missense reversion of a chain-terminator mutation in Neurospora. Genetics. 1977; 86(2 Pt. 1):261-74. PMC: 1213676. View

12.

Reinert W, Marzluf G . Genetic and metabolic control of the purine catabolic enzymes of Neurospora crasse. Mol Gen Genet. 1975; 139(1):39-55. DOI: 10.1007/BF00267994. View

13.

Jarai G, Marzluf G . Analysis of conventional and in vitro generated mutants of nmr, the negatively acting nitrogen regulatory gene of Neurospora crassa. Mol Gen Genet. 1990; 222(2-3):233-40. DOI: 10.1007/BF00633823. View

14.

Palacios R, Campomanes M, Quinto C . Neurospora crassa glutamine synthetase. Translation of specific messenger ribonucleic acid in a cell-free system derived from rabbit reticulocytes. J Biol Chem. 1977; 252(9):3028-34. View

15.

SORGER G, Debanne M, Davies J . Effect of nitrate on the synthesis and decay of nitrate reductase of Neurospora. Biochem J. 1974; 140(3):395-403. PMC: 1168016. DOI: 10.1042/bj1400395. View

16.

Vichido I, Mora Y, Quinto C, Palacios R, Mora J . Nitrogen regulation of glutamine synthetase in Neurospora crassa. J Gen Microbiol. 1978; 106(2):251-9. DOI: 10.1099/00221287-106-2-251. View

17.

Calderon J, Martinez L, Mora J . Isolation and characterization of a Neurospora crassa mutant altered in the alpha polypeptide of glutamine synthetase. J Bacteriol. 1990; 172(9):4996-5000. PMC: 213155. DOI: 10.1128/jb.172.9.4996-5000.1990. View

18.

Calderon J, Morett E, Mora J . Omega-amidase pathway in the degradation of glutamine in Neurospora crassa. J Bacteriol. 1985; 161(2):807-9. PMC: 214962. DOI: 10.1128/jb.161.2.807-809.1985. View

19.

Fincham J, Baron A . The molecular basis of an osmotically reparable mutant of Neurospora crassa producing unstable glutamate dehydrogenase. J Mol Biol. 1977; 110(4):627-42. DOI: 10.1016/s0022-2836(77)80081-8. View

20.

Davila G, Brom S, Mora Y, Palacios R, Mora J . Genetic and biochemical characterization of glutamine synthetase from Neurospora crassa glutamine auxotrophs and their revertants. J Bacteriol. 1983; 156(3):993-1000. PMC: 217941. DOI: 10.1128/jb.156.3.993-1000.1983. View