Polymerase Chain Reaction and Sequencing for Typing Rhinovirus RNA

Overview

Journal J Med Virol

Specialty Microbiology

Date 1994 Dec 1

PMID 7897363

Citations 26

Authors

J Mori

J P Clewley

Affiliations

Soon will be listed here.

Abstract

Primers were designed and tested for their ability to distinguish rhinoviruses from enteroviruses. A primer set derived from the 5'-UTR/VP coding region junction was able to amplify all the rhinovirus serotypes tested. Enteroviruses were either not amplified by these primer pairs or produced a band of larger size that could easily be discriminated from the rhinovirus-specific product. In contrast, primers embedded in the 5'-UTR region alone were able to amplify both rhinovirus and enterovirus RNA. It is shown that rhinoviruses could be specifically typed by sequencing the amplicon derived from the 5'-UTR set. The sequences of the 5'-UTR region of ten previously unsequenced rhinoviruses were derived. The sequences obtained cluster into two groups: 1B, 41, 15, 30, 63, 31, 56, and 44; and 17, 69, and 70. Amplicons from serotypes 17,69, and 70 also group by sequence with the equivalent region of HRV14 from the genetic database, while the others group with 2 and 89.

Citing Articles

Degenerate PCR primer design for the specific identification of rhinovirus C.

Nam Y, Lee U, Choi H, Lee K, Kim N, Jang Y J Virol Methods. 2014; 214:15-24.

PMID: 25483126 PMC: 7113658. DOI: 10.1016/j.jviromet.2014.10.021.

Population structure and evolution of Rhinoviruses.

Waman V, Kolekar P, Kale M, Kulkarni-Kale U PLoS One. 2014; 9(2):e88981.

PMID: 24586469 PMC: 3929619. DOI: 10.1371/journal.pone.0088981.

Rhinovirus detection using different PCR-based strategies.

Silva E, Watanabe A, Carraro E, Perosa A, Granato C, Bellei N Braz J Microbiol. 2013; 43(2):739-43.

PMID: 24031885 PMC: 3768804. DOI: 10.1590/S1517-83822012000200038.

Human rhinoviruses.

Jacobs S, Lamson D, St George K, Walsh T Clin Microbiol Rev. 2013; 26(1):135-62.

PMID: 23297263 PMC: 3553670. DOI: 10.1128/CMR.00077-12.

Virological investigation of hand, foot, and mouth disease in a tertiary care center in South India.

Vijayaraghavan P, Chandy S, Selvaraj K, Pulimood S, Abraham A J Glob Infect Dis. 2012; 4(3):153-61.

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