A Carbon Starvation Survival Gene of Pseudomonas Putida is Regulated by Sigma 54

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1995 Apr 1

PMID 7896711

Citations 13

Authors

Y Kim

L S Watrud

A Matin

Affiliations

Soon will be listed here.

Abstract

By using mini-Tn5 transposon mutagenesis, two mutants of Pseudomonas putida ATCC 12633 were isolated which showed a marked increase in their sensitivity to carbon starvation; these mutants are presumably affected in the Pex type of proteins that P. putida induces upon carbon starvation (M. Givskov, L. Eberl, and S. Molin, J. Bacteriol. 176:4816-4824, 1994). The affected genes in our mutants were induced about threefold upon carbon starvation. The promoter region of the starvation gene in the mutant MK107 possessed a strong sigma 54-type-promoter sequence, and deletion analysis suggested that this was the major promoter regulating expression; this was confirmed by transcript mapping in rpoN+ and rpoN mutant backgrounds. The deletion analysis implicated a sequence upstream of the sigma 54 promoter, as well as a region downstream of the transcription start site, in the functioning of the promoter. Two sigma 70-type Pribnow boxes were also detected in the promoter region, but their transcriptional activity in the wild type was very weak. However, in a sigma 54-deficient background, these promoters became stronger. The mechanism and possible physiological role of this phenomenon and the possibility that the sequence upstream of the sigma 54 promoter may have a role in carbon sensing are discussed.

Citing Articles

Transcriptional organization, regulation and functional analysis of flhF and fleN in Pseudomonas putida.

Navarrete B, Leal-Morales A, Serrano-Ron L, Sarrio M, Jimenez-Fernandez A, Jimenez-Diaz L PLoS One. 2019; 14(3):e0214166.

PMID: 30889223 PMC: 6424431. DOI: 10.1371/journal.pone.0214166.

Involvement of RpoN in regulating bacterial arsenite oxidation.

Kang Y, Bothner B, Rensing C, McDermott T Appl Environ Microbiol. 2012; 78(16):5638-45.

PMID: 22660703 PMC: 3406167. DOI: 10.1128/AEM.00238-12.

Uptake of carbon monoxide and hydrogen at environmentally relevant concentrations by mycobacteria.

King G Appl Environ Microbiol. 2003; 69(12):7266-72.

PMID: 14660375 PMC: 310020. DOI: 10.1128/AEM.69.12.7266-7272.2003.

Inactivation of gltB abolishes expression of the assimilatory nitrate reductase gene (nasB) in Pseudomonas putida KT2442.

Eberl L, Ammendola A, Rothballer M, Givskov M, Sternberg C, Kilstrup M J Bacteriol. 2000; 182(12):3368-76.

PMID: 10852866 PMC: 101894. DOI: 10.1128/JB.182.12.3368-3376.2000.

Purification to homogeneity and characterization of a novel Pseudomonas putida chromate reductase.

Park C, Keyhan M, Wielinga B, Fendorf S, Matin A Appl Environ Microbiol. 2000; 66(5):1788-95.

PMID: 10788340 PMC: 101413. DOI: 10.1128/AEM.66.5.1788-1795.2000.

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