Chemical Synthesis, Expression and Mutagenesis of a Gene Encoding Beta-cryptogein, an Elicitin Produced by Phytophthora Cryptogea

Overview

Journal Plant Mol Biol

Date 1995 Feb 1

PMID 7894020

Citations 10

Authors

M J ODonohue

H Gousseau

J C Huet

D Tepfer

J C Pernollet

Affiliations

Soon will be listed here.

Abstract

Elicitins are 10 kDa holoproteins secreted by Phytophthora fungi, that elicit an incompatible hypersensitive reaction, leading to resistance against fungal and bacterial plant pathogens. Comparison of primary sequences of alpha-elicitins and beta-elicitins indicated several potential necrotic activity-determining residues. All of the highly necrotic beta-elicitins have a hydrophilic residue (usually lysine) at position 13, whereas in the less necrotic alpha-elicitins this residue is replaced by a valine. Here, we report the synthesis and expression of a gene encoding a highly necrotic elicitin, beta-cryptogein, and we show that the substitution of Lys-13 of this recombinant protein by a valine leads to a drastic alteration to the necrotic activity of the recombinant protein.

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