Semi-microtechnique for the Biochemical Characterization of Anaerobic Bacteria

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1976 Oct 1

PMID 789392

Citations 3

Authors

J R Morgan

P Y Liu

J A Smith

Affiliations

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Abstract

A semi-micromethod is described for characterizing anaerobic bacteria by substrate utilization. Small volumes of individual substrates were placed in the wells of plastic microtiter trays. When heavy inocula and a colorimetric indicator were used, complete results were available after 30 h of incubation. Microtiter trays containing a range of substrates can be stored at -20 degrees C. The method is accurate, economical, and convenient for use in a hospital microbiology laboratory.

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Simple disk technique for carbohydrate fermentation and esculin hydrolysis testing of anaerobic gram-negative bacilli.

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References

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Wilkins T, Walker C, Moore W . Micromethod for identification of anaerobic bacteria: design and operation of apparatus. Appl Microbiol. 1975; 30(5):831-7. PMC: 187280. DOI: 10.1128/am.30.5.831-837.1975. View

Schreckenberger P, Blazevic D . Rapid methods for biochemical testing of anaerobic bacteria. Appl Microbiol. 1974; 28(5):759-62. PMC: 186820. DOI: 10.1128/am.28.5.759-762.1974. View