A 7-base-pair Sequence Protects DNA from Exonucleolytic Degradation in Lactococcus Lactis

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1995 Mar 14

PMID 7892255

Citations 22

Authors

I Biswas

E Maguin

S D Ehrlich

A Gruss

Affiliations

Soon will be listed here.

Abstract

Linear DNA molecules are subject to degradation by various exonucleases in vivo unless their ends are protected. It has been demonstrated that a specific 8-bp sequence, 5'-GCTGGTGG-3', named Chi, can protect linear double-stranded DNA from the major Escherichia coli exonuclease RecBCD. Chi protects linear replication products of rolling-circle plasmids from RecBCD degradation in vivo, in agreement with observations in vitro. A unique 7-bp sequence, 5'-GCGCGTG-3', is shown to protect similar replication products from degradation in Lactococcus lactis strains but not in more distantly related Gram-positive bacteria. The properties of this sequence in L. lactis correspond to those of a Chi site. Linear plasmid replication products have been detected in numerous prokaryotes, suggesting the widespread existence of short species-specific sequences that preserve linear DNA from extensive degradation by host cell exonucleases.

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