Myosin-actin Interaction Plays an Important Role in Human Immunodeficiency Virus Type 1 Release from Host Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1995 Mar 14

PMID 7892219

Citations 66

Authors

H Sasaki

M Nakamura

T Ohno

Y Matsuda

Y Yuda

Y Nonomura

Affiliations

Soon will be listed here.

Abstract

We examined the potential role of myosin and actin in the release of human immunodeficiency virus type 1 (HIV-1) from infected cells. Wortmannin (100 nM to 5 microM), an effective inhibitor of myosin light chain kinase, blocked the release of HIV-1 from infected T-lymphoblastoid and monocytoid cells in a concentration-dependent manner. Cytochalasin D, a reagent that disrupts the equilibrium between monomeric and polymeric actin, also partially inhibited the release of HIV-1 from the infected cells. At the budding stage, myosin and HIV-1 protein were detected in the same areas on the plasma membrane by using dual-label immunofluorescence microscopy and immunoelectron microscopy. In the presence of 5 microM wortmannin, viral components were observed on the plasma membrane by using immunofluorescence microscopy and electron microscopy, implying that wortmannin did not disturb the transport of viral proteins to the plasma membrane but rather inhibited budding.

Citing Articles

HIV-1 diverts cortical actin for particle assembly and release.

Dibsy R, Bremaud E, Mak J, Favard C, Muriaux D Nat Commun. 2023; 14(1):6945.

PMID: 37907528 PMC: 10618566. DOI: 10.1038/s41467-023-41940-0.

Actin-regulated Siglec-1 nanoclustering influences HIV-1 capture and virus-containing compartment formation in dendritic cells.

Gutierrez-Martinez E, Benet Garrabe S, Mateos N, Erkizia I, Nieto-Garai J, Lorizate M Elife. 2023; 12.

PMID: 36940134 PMC: 10065798. DOI: 10.7554/eLife.78836.

Nonmuscle myosin IIA promotes the internalization of influenza A virus and regulates viral polymerase activity through interacting with nucleoprotein in human pulmonary cells.

Chen J, Liu J, Chen Z, Feng D, Zhu C, Fan J Virol Sin. 2022; 38(1):128-141.

PMID: 36509386 PMC: 10006312. DOI: 10.1016/j.virs.2022.12.002.

Characterization of Extracellular Vesicles Secreted in Lentiviral Producing HEK293SF Cell Cultures.

Minh A, Star A, Stupak J, Fulton K, Haqqani A, Gelinas J Viruses. 2021; 13(5).

PMID: 33946875 PMC: 8145507. DOI: 10.3390/v13050797.

Comparative Genomics and Integrated Network Approach Unveiled Undirected Phylogeny Patterns, Co-mutational Hot Spots, Functional Cross Talk, and Regulatory Interactions in SARS-CoV-2.

Gupta V, Haider S, Verma M, Singhvi N, Ponnusamy K, Malik M mSystems. 2021; 6(1).

PMID: 33622851 PMC: 8573956. DOI: 10.1128/mSystems.00030-21.

References

Korn E . Actin polymerization and its regulation by proteins from nonmuscle cells. Physiol Rev. 1982; 62(2):672-737. DOI: 10.1152/physrev.1982.62.2.672. View

Kumakura K, Sasaki K, Sakurai T, Ohara-Imaizumi M, Misonou H, Nakamura S . Essential role of myosin light chain kinase in the mechanism for MgATP-dependent priming of exocytosis in adrenal chromaffin cells. J Neurosci. 1994; 14(12):7695-703. PMC: 6576909. View

Hoffman A, Banapour B, Levy J . Characterization of the AIDS-associated retrovirus reverse transcriptase and optimal conditions for its detection in virions. Virology. 1985; 147(2):326-35. DOI: 10.1016/0042-6822(85)90135-7. View

Bohn W, RUTTER G, Hohenberg H, Mannweiler K, Nobis P . Involvement of actin filaments in budding of measles virus: studies on cytoskeletons of infected cells. Virology. 1986; 149(1):91-106. DOI: 10.1016/0042-6822(86)90090-5. View

Twentyman P, LUSCOMBE M . A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity. Br J Cancer. 1987; 56(3):279-85. PMC: 2002206. DOI: 10.1038/bjc.1987.190. View

Cooper J . Effects of cytochalasin and phalloidin on actin. J Cell Biol. 1987; 105(4):1473-8. PMC: 2114638. DOI: 10.1083/jcb.105.4.1473. View

Moyer S, Baker S, Horikami S . Host cell proteins required for measles virus reproduction. J Gen Virol. 1990; 71 ( Pt 4):775-83. DOI: 10.1099/0022-1317-71-4-775. View

Nakanishi S, Yamada K, Iwahashi K, Kuroda K, Kase H . KT5926, a potent and selective inhibitor of myosin light chain kinase. Mol Pharmacol. 1990; 37(4):482-8. View

Geelen J, Goudsmit J . Virus-host interactions in human immunodeficiency virus infection. Prog Med Virol. 1991; 38:27-41. View

10.

Vaishnav Y, Wong-Staal F . The biochemistry of AIDS. Annu Rev Biochem. 1991; 60:577-630. DOI: 10.1146/annurev.bi.60.070191.003045. View

11.

Hashimoto Y, Nakayama T, Teramoto T, Kato H, Watanabe T, Kinoshita M . Potent and preferential inhibition of Ca2+/calmodulin-dependent protein kinase II by K252a and its derivative, KT5926. Biochem Biophys Res Commun. 1991; 181(1):423-9. DOI: 10.1016/s0006-291x(05)81436-6. View

12.

Brenner S, Korn E . Substoichiometric concentrations of cytochalasin D inhibit actin polymerization. Additional evidence for an F-actin treadmill. J Biol Chem. 1979; 254(20):9982-5. View

13.

FLANAGAN M, Lin S . Cytochalasins block actin filament elongation by binding to high affinity sites associated with F-actin. J Biol Chem. 1980; 255(3):835-8. View

14.

Brown S, Spudich J . Cytochalasin inhibits the rate of elongation of actin filament fragments. J Cell Biol. 1979; 83(3):657-62. PMC: 2110509. DOI: 10.1083/jcb.83.3.657. View

15.

Lin D, Tobin K, Grumet M, Lin S . Cytochalasins inhibit nuclei-induced actin polymerization by blocking filament elongation. J Cell Biol. 1980; 84(2):455-60. PMC: 2110542. DOI: 10.1083/jcb.84.2.455. View

16.

Ohno T, Terada M, Yoneda Y, Shea K, Chambers R, Stroka D . A broadly neutralizing monoclonal antibody that recognizes the V3 region of human immunodeficiency virus type 1 glycoprotein gp120. Proc Natl Acad Sci U S A. 1991; 88(23):10726-9. PMC: 53003. DOI: 10.1073/pnas.88.23.10726. View

17.

Nakanishi S, Kakita S, Takahashi I, Kawahara K, Tsukuda E, Sano T . Wortmannin, a microbial product inhibitor of myosin light chain kinase. J Biol Chem. 1992; 267(4):2157-63. View

18.

Kitani S, Teshima R, Morita Y, Ito K, Matsuda Y, Nonomura Y . Inhibition of IgE-mediated histamine release by myosin light chain kinase inhibitors. Biochem Biophys Res Commun. 1992; 183(1):48-54. DOI: 10.1016/0006-291x(92)91607-r. View

19.

Ohara-Imaizumi M, Sakurai T, Nakamura S, Nakanishi S, Matsuda Y, Muramatsu S . Inhibition of Ca(2+)-dependent catecholamine release by myosin light chain kinase inhibitor, wortmannin, in adrenal chromaffin cells. Biochem Biophys Res Commun. 1992; 185(3):1016-21. DOI: 10.1016/0006-291x(92)91728-9. View

20.

Gottlieb T, Ivanov I, Adesnik M, Sabatini D . Actin microfilaments play a critical role in endocytosis at the apical but not the basolateral surface of polarized epithelial cells. J Cell Biol. 1993; 120(3):695-710. PMC: 2119548. DOI: 10.1083/jcb.120.3.695. View