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ColE Plasmid Replication in DNA Polymerase I-deficient Strains of Escherichia Coli

Overview
Journal Mol Gen Genet
Specialties Genetics
Molecular Biology
Date 1976 Sep 23
PMID 787767
Citations 19
Authors
W Tacon
D Sherratt
Affiliations
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Abstract

Replication of the non-conjugative plasmids ColE1, ColE2 and Col3 has been examined in a number of DNA polymerase I-deficient strains, two of which contain the amber mutation polA1 along with either of two temperature-sensitive supF amber suppressors. These latter two strains produce reduced amounts of DNA polymerase I polymerizing activity of similar, if not identical properties to that produced by polA+ strains. Our results indicate that the ColE plasmids require different amounts of DNA polymerase I for stable plasmid maintenance. Moreover whereas all three plasmids are maintained in a strain defective in the 5' leads to 3' exonuclease activity of DNA polymerase I, ColE2 and ColE3 are not stably maintained between 30 degrees and 43 degrees in a number of DNA POLYMERASE I-deficient strains that are temperature-sensitive for ColE1 replication.

Citing Articles

Roles of DNA polymerase I in leading and lagging-strand replication defined by a high-resolution mutation footprint of ColE1 plasmid replication.

Allen J, Simcha D, Ericson N, Alexander D, Marquette J, Van Biber B Nucleic Acids Res. 2011; 39(16):7020-33.

PMID: 21622658 PMC: 3167613. DOI: 10.1093/nar/gkr157.

Distinct functions of the two specificity determinants in replication initiation of plasmids ColE2-P9 and ColE3-CA38.

Aoki K, Shinohara M, Itoh T J Bacteriol. 2007; 189(6):2392-400.

PMID: 17237180 PMC: 1899376. DOI: 10.1128/JB.01695-06.

Specific interaction between the initiator protein (Rep) and origin of plasmid ColE2-P9.

Han M, Yagura M, Itoh T J Bacteriol. 2006; 189(3):1061-71.

PMID: 17098894 PMC: 1797306. DOI: 10.1128/JB.01455-06.

Anatomy of the replication origin of plasmid ColE2-P9.

Yagura M, Nishio S, Kurozumi H, Wang C, Itoh T J Bacteriol. 2006; 188(3):999-1010.

PMID: 16428404 PMC: 1347323. DOI: 10.1128/JB.188.3.999-1010.2006.

DNA polymerase I in constitutive stable DNA replication in Escherichia coli.

Kogoma T, Maldonado R J Bacteriol. 1997; 179(7):2109-15.

PMID: 9079893 PMC: 178944. DOI: 10.1128/jb.179.7.2109-2115.1997.

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