Phage T4 Infection Restricts RRNA Synthesis by E. Coli RNA Polymerase

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1976 Sep 23

PMID 787764

Citations 3

Authors

F E Baralle

A Travers

Affiliations

Soon will be listed here.

Abstract

RNA polymerase from T4 infected cells supplemented with E. coli sigma polypeptide has a lower affinity for rRNA promoters than RNA polymerase from uninfected cells. The pattern of transcription by the phage modified polymerase is qualitatively similar to that of the vegetative polymerase in the presence of ppGpp. We suggest that E. coli polymerase holoenzyme normally exists in at least two conformational states, one with a high affinity for rRNA promoters and another with a low affinity, and that T4 infection stabilises the low affinity form.

Citing Articles

Changes in the promoter range of RNA polymerase resulting from bacteriophage T4-induced modification of core enzyme.

Goldfarb A Proc Natl Acad Sci U S A. 1981; 78(6):3454-8.

PMID: 7022450 PMC: 319587. DOI: 10.1073/pnas.78.6.3454.

Shutoff of host macromolecular synthesis after T-even bacteriophage infection.

Koerner J, Snustad D Microbiol Rev. 1979; 43(2):199-223.

PMID: 390354 PMC: 281471. DOI: 10.1128/mr.43.2.199-223.1979.

Slow switchover from host RNA synthesis to bacteriophage RNA synthesis after infection of Escherichia coli with a T4 mutant defective in the bacteriophage T4-induced unfolding of the host nucleoid.

Tigges M, Bursch C, Snustad D J Virol. 1977; 24(3):775-85.

PMID: 201776 PMC: 515999. DOI: 10.1128/JVI.24.3.775-785.1977.

References

Otsubo E, Soll L, Deonier R, Lee H, Davidson N . Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. VIII. The structure of bacteriophage phi 80d-3ilv+su+7, including the mapping of the ribosomal RNA genes. J Mol Biol. 1974; 89(4):631-46. DOI: 10.1016/0022-2836(74)90040-0. View

Adesnik M, Levinthal C . RNA metabolism in T4-infected Escherichia coli. J Mol Biol. 1970; 48(2):187-208. DOI: 10.1016/0022-2836(70)90156-7. View

Travers A . RNA polymerase--promoter interactions: some general principles. Cell. 1974; 3(2):97-104. DOI: 10.1016/0092-8674(74)90112-3. View

Schachner M, Seifert W, Zillig W . A correlation of changes in host and T 4 bacteriophage specific RNA synthesis with changes of DNA-dependent RNA polymerase in Escherichia coli infected with bacteriophage T 4 . Eur J Biochem. 1971; 22(4):520-8. DOI: 10.1111/j.1432-1033.1971.tb01572.x. View

Landy A, Spiegelman S . Exhaustive hybridization and its application to an analysis of the ribonucleic acid synthesized in T4-infected cells. Biochemistry. 1968; 7(2):585-91. DOI: 10.1021/bi00842a011. View

Horvitz H . Polypeptide bound to the host RNA polymerase is specified by T4 control gene 33. Nat New Biol. 1973; 244(135):137-40. DOI: 10.1038/newbio244137a0. View

CASHEL M . The control of ribonucleic acid synthesis in Escherichia coli. IV. Relevance of unusual phosphorylated compounds from amino acid-starved stringent strains. J Biol Chem. 1969; 244(12):3133-41. View

Stevens A . Deoxyribonucleic acid dependent ribonucleic acid polymerases from two T4 phage-infected systems. Biochemistry. 1974; 13(3):493-503. DOI: 10.1021/bi00700a015. View

Ratner D . Letter to the editor: Bacteriophage T4 transcriptional control gene 55 codes for a protein bound to Escherichia coli RNA polymerase. J Mol Biol. 1974; 89(4):803-7. DOI: 10.1016/0022-2836(74)90054-0. View

10.

Travers A . Modulation of RNA polymerase specificity by ppGpp. Mol Gen Genet. 1976; 147(2):225-32. DOI: 10.1007/BF00267575. View

11.

Nomura M, Okamoto K, Asano K . RNA metabolism in Escherichia coli infected with bacteriophage T4. Inhibition of host ribosomal and soluble RNA synthesis by phage and effect of chloromycetin. J Mol Biol. 1962; 4:376-87. DOI: 10.1016/s0022-2836(62)80018-7. View

12.

CHAMBERLIN M . The selectivity of transcription. Annu Rev Biochem. 1974; 43(0):721-75. DOI: 10.1146/annurev.bi.43.070174.003445. View

13.

Haseltine W . In vitro transcription of Escherichia coli ribosomal RNA genes. Nature. 1972; 235(5337):329-33. DOI: 10.1038/235329a0. View

14.

Walter G, Seifert W, Zillig W . Modified DNA-dependent RNA polymerase from E. coli infected with bacteriophage T4. Biochem Biophys Res Commun. 1968; 30(3):240-7. DOI: 10.1016/0006-291x(68)90441-5. View

15.

Stevens A . New small polypeptides associated with DNA-dependent RNA polymerase of Escherichia coli after infection with bacteriophage T4. Proc Natl Acad Sci U S A. 1972; 69(3):603-7. PMC: 426516. DOI: 10.1073/pnas.69.3.603. View

16.

Goff C . Chemical structure of a modification of the Escherichia coli ribonucleic acid polymerase alpha polypeptides induced by bacteriophage T4 infection. J Biol Chem. 1974; 249(19):6181-90. View

17.

Mailhammer R, Yang H, Reiness G, Zubay G . Effects of bacteriophage T4-induced modification of Escherichia coli RNA polymerase on gene expression in vitro. Proc Natl Acad Sci U S A. 1975; 72(12):4928-32. PMC: 388846. DOI: 10.1073/pnas.72.12.4928. View

18.

Horvitz H . Bacteriophage T4 mutants deficient in alteration and modification of the Escherichia coli RNA polymerase. J Mol Biol. 1974; 90(4):739-50. DOI: 10.1016/0022-2836(74)90537-3. View