Analysis of the Introns in Genes Encoding Small G Proteins

Overview

Journal Curr Genet

Specialty Genetics

Date 1994 Nov 1

PMID 7874745

Citations 5

Authors

W Dietmaier

S FABRY

Affiliations

Soon will be listed here.

Abstract

Because all small G proteins (SGPs) possess a very similar array of structural and functional domains, they are obvious candidates for examining the relationships postulated to exist between the exon-intron structure of genes and the domain structure of the encoded proteins. To address this issue, and to possibly gain insight into the evolution of their introns, we have analyzed positions, sizes, and sequences of 125 introns from 28 SGP genes. These introns were found to be distributed in 60 different locations throughout the aligned sequences, with a preference for the 5'-half of the genes. More than 50% of the positions were found to be shared by two or more genes, and genes encoding SGPs of very similar amino acid sequence (i.e., isotypes) in quite closely related species tend to have most, or all, of their introns in identical locations, indicating a common evolutionary origin (homologous introns). However, with few exceptions, no statistically significant sequence similarity or common folding motif was found between homologous intron pairs. Only three intron positions are shared between members of distantly related SGP subfamilies. These three potentially ancient intron locations fall between regions encoding alpha-helices or beta-sheets, but two of them interrupt regions encoding known functional (guanosine-nucleotide-binding) modules. Intron positions that are occupied only in single genes, or in genes encoding very similar SGPs, do not show any preferential distribution with respect to regions encoding structural or functional motifs.(ABSTRACT TRUNCATED AT 250 WORDS)

Citing Articles

The Dictyostelium discoideum family of Rho-related proteins.

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Intron loss and gain during evolution of the catalase gene family in angiosperms.

Frugoli J, McPeek M, Thomas T, McClung C Genetics. 1998; 149(1):355-65.

PMID: 9584109 PMC: 1460146. DOI: 10.1093/genetics/149.1.355.

Intron "sliding" and the diversity of intron positions.

Stoltzfus A, Logsdon Jr J, Palmer J, Doolittle W Proc Natl Acad Sci U S A. 1997; 94(20):10739-44.

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GTP-binding proteins in plants: new members of an old family.

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Structure-function analysis of small G proteins from Volvox and Chlamydomonas by complementation of Saccharomyces cerevisiae YPT/SEC mutations.

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