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Six-hour Pigmentation Test for the Identification of Cryptococcus Neoformans

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1976 Aug 1
PMID 787003
Citations 19
Authors
R L Hopfer
D Groschel
Affiliations
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Abstract

Cryptococcus neoformans colonies can be identified within 6 h using paper disks containing caffeic acid and ferric citrate. Indentification is based on the development of a dark brown pigment. Saprophytic Cryptococcus species and common clinically isolated yeasts do not develop the brown color. The concentration of the reagents and the method of storage of the impregnated paper disks are critical for the rapid and specific development of the pigment.

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References
1.
Hopfer R, Blank F . Caffeic acid-containing medium for identification of Cryptococcus neoformans. J Clin Microbiol. 1976; 2(2):115-20. PMC: 274144. View
2.
Korth H, Pulverer G . Pigment formation for differentiating Cryptococcus neoformans from Candida albicans. Appl Microbiol. 1971; 21(3):541-2. PMC: 377219. DOI: 10.1128/am.21.3.541-542.1971. View
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Shaw C, KAPICA L . Production of diagnostic pigment by phenoloxidase activity of cryptococcus neoformans. Appl Microbiol. 1972; 24(5):824-30. PMC: 380670. DOI: 10.1128/am.24.5.824-830.1972. View
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STRACHAN A, Yu R, Blank F . Pigment production of Cryptococcus neoformans grown with extracts of Guizotia abyssinica. Appl Microbiol. 1971; 22(3):478-9. PMC: 376340. DOI: 10.1128/am.22.3.478-479.1971. View
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Breyer D, Staib F, Senska M, BLISSE A . [Suitability of the O-diphenol oxidase test using catechol for the differentiation of Cryptococcus neoformans]. Zentralbl Bakteriol Orig A. 1972; 222(4):540-3. View