The Relationship Between Size and Maturation in Vitro in the Unstimulated Human Oocyte

Overview

Journal Fertil Steril

Specialty Reproductive Medicine

Date 1995 Feb 1

PMID 7843451

Citations 14

Authors

K L Durinzi

E M Saniga

S E Lanzendorf

Affiliations

Soon will be listed here.

Abstract

Objective: To determine if the size of human oocytes at collection from unstimulated ovaries is related to their ability to resume meiosis and undergo maturation in vitro.

Design: A comparative study of oocyte diameter at collection.

Setting: Department of Obstetrics and Gynecology, Northwestern University Medical School.

Patients: Women age 25 to 39 years of age undergoing gynecological procedures yielding oophorectomy specimens.

Intervention: Oocytes obtained from ovarian tissue were cultured in Ham's F-10 and fetal bovine serum for 72 hours and observed two times per day.

Main Outcome Measure: The oocytes ability to resume meiosis and complete maturation based on their diameter at collection.

Results: Chi-squared analysis revealed a significant difference in oocytes measuring 86 to 105 microns versus those measuring 106 to 125 microns.

Conclusion: The unstimulated human oocyte appears to have a size-dependent ability to resume meiosis and complete maturation.

Citing Articles

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Oocyte Competence, Embryological Outcomes and miRNA Signature of Different Sized Follicles from Poor Responder Patients.

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Oocyte diameter predicts the maturation rate of human immature oocytes collected ex vivo.

Pors S, Nikiforov D, Cadenas J, Ghezelayagh Z, Wakimoto Y, Jara L J Assist Reprod Genet. 2022; 39(10):2209-2214.

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Enhancing Oocyte Competence With Milrinone as a Phosphodiesterase 3A Inhibitor to Improve the Development of Porcine Cloned Embryos.

Roy P, Qamar A, Tanga B, Fang X, Kim G, Bang S Front Cell Dev Biol. 2021; 9:647616.

PMID: 33996810 PMC: 8120234. DOI: 10.3389/fcell.2021.647616.

Unscrambling the oocyte and the egg: clarifying terminology of the female gamete in mammals.

Duncan F, Schindler K, Schultz R, Blengini C, Stein P, Stricker S Mol Hum Reprod. 2020; 26(11):797-800.

PMID: 33022047 PMC: 7648930. DOI: 10.1093/molehr/gaaa066.