Purification, Characterization and Reconstitution of CMP-N-acetylneuraminate Hydroxylase from Mouse Liver

Overview

Journal Glycoconj J

Publisher Springer

Specialties Biochemistry
Endocrinology

Date 1994 Jun 1

PMID 7841794

Citations 2

Authors

P Schneckenburger

L Shaw

R Schauer

Affiliations

Soon will be listed here.

Abstract

CMP-N-acetylneuraminate hydroxylase was isolated from mouse liver high speed supernatant with a yield of 0.4% and an apparent 1000-fold purification. The enzyme is a monomeric protein with a molecular weight of 66 kDa, as determined by gel filtration and SDS-PAGE. The hydroxylase system was reconstituted with Triton X-100-solubilized mouse liver microsomes and purified soluble or microsomal forms of cytochrome b5 reductase and cytochrome b5. The systems were characterized in detail and kinetic parameters for each system were determined.

Citing Articles

Exploration of the Sialic Acid World.

Schauer R, Kamerling J Adv Carbohydr Chem Biochem. 2018; 75:1-213.

PMID: 30509400 PMC: 7112061. DOI: 10.1016/bs.accb.2018.09.001.

A comprehensive phylogenetic analysis of Rieske and Rieske-type iron-sulfur proteins.

Schmidt C, Shaw L J Bioenerg Biomembr. 2001; 33(1):9-26.

PMID: 11460929 DOI: 10.1023/a:1005616505962.

References

Shaw L, Schauer R . Detection of CMP-N-acetylneuraminic acid hydroxylase activity in fractionated mouse liver. Biochem J. 1989; 263(2):355-63. PMC: 1133437. DOI: 10.1042/bj2630355. View

Muchmore E . Developmental sialic acid modifications in rat organs. Glycobiology. 1992; 2(4):337-43. DOI: 10.1093/glycob/2.4.337. View

Lever M . Peroxides in detergents as interfering factors in biochemical analysis. Anal Biochem. 1977; 83(1):274-84. DOI: 10.1016/0003-2697(77)90536-x. View

Shaw L, Schneckenburger P, Carlsen J, Christiansen K, Schauer R . Mouse liver cytidine-5'-monophosphate-N-acetylneuraminic acid hydroxylase. Catalytic function and regulation. Eur J Biochem. 1992; 206(1):269-77. DOI: 10.1111/j.1432-1033.1992.tb16925.x. View

MARGOLIASH E, FROHWIRT N . Spectrum of horse-heart cytochrome c. Biochem J. 1959; 71(3):570-2. PMC: 1196833. DOI: 10.1042/bj0710570. View

Shaw L, Yousefi S, Dennis J, Schauer R . CMP-N-acetylneuraminic acid hydroxylase activity determines the wheat germ agglutinin-binding phenotype in two mutants of the lymphoma cell line MDAY-D2. Glycoconj J. 1991; 8(5):434-41. DOI: 10.1007/BF00731295. View

BERMAN M, Adnams C, Ivanetich K, KENCH J . Autoxidation of soluble trypsin-cleaved microsomal ferrocytochrome b5 and formation of superoxide radicals. Biochem J. 1976; 157(1):237-46. PMC: 1163838. DOI: 10.1042/bj1570237. View

Kozutsumi Y, Kawano T, Kawasaki H, Suzuki K, Yamakawa T, Suzuki A . Reconstitution of CMP-N-acetylneuraminic acid hydroxylation activity using a mouse liver cytosol fraction and soluble cytochrome b5 purified from horse erythrocytes. J Biochem. 1991; 110(3):429-35. DOI: 10.1093/oxfordjournals.jbchem.a123598. View

Higashi H, Hirabayashi Y, Fukui Y, Naiki M, Matsumoto M, Ueda S . Characterization of N-glycolylneuraminic acid-containing gangliosides as tumor-associated Hanganutziu-Deicher antigen in human colon cancer. Cancer Res. 1985; 45(8):3796-802. View

10.

Spatz L, STRITTMATTER P . A form of reduced nicotinamide adenine dinucleotide-cytochrome b 5 reductase containing both the catalytic site and an additional hydrophobic membrane-binding segment. J Biol Chem. 1973; 248(3):793-9. View

11.

Tajima S, Enomoto K, Sato R . Nature of tryptic attack on cytochrome b5 and further evidence for the two-domain structure of the cytochrome molecule. J Biochem. 1978; 84(6):1573-86. DOI: 10.1093/oxfordjournals.jbchem.a132283. View

12.

Higashi H, Ikuta K, Ueda S, Kato S, Hirabayashi Y, Matsumoto M . Characterization of N-glycolyneuraminic acid-containing glycosphingolipids from a Marek's disease lymphoma-derived chicken cell line, MSB1, as tumor-associated heterophile Hanganutziu-Deicher antigens. J Biochem. 1984; 95(3):785-94. DOI: 10.1093/oxfordjournals.jbchem.a134670. View

13.

Shaw L, Schauer R . The biosynthesis of N-glycoloylneuraminic acid occurs by hydroxylation of the CMP-glycoside of N-acetylneuraminic acid. Biol Chem Hoppe Seyler. 1988; 369(6):477-86. DOI: 10.1515/bchm3.1988.369.1.477. View

14.

Lepers A, Shaw L, Schneckenburger P, Cacan R, Verbert A, Schauer R . A study on the regulation of N-glycoloylneuraminic acid biosynthesis and utilization in rat and mouse liver. Eur J Biochem. 1990; 193(3):715-23. DOI: 10.1111/j.1432-1033.1990.tb19391.x. View

15.

Reuter G, Schauer R . Determination of sialic acids. Methods Enzymol. 1994; 230:168-99. DOI: 10.1016/0076-6879(94)30012-7. View

16.

Passon P, HULTQUIST D . Soluble cytochrome b 5 reductase from human erythrocytes. Biochim Biophys Acta. 1972; 275(1):62-73. DOI: 10.1016/0005-2728(72)90024-2. View

17.

Kawano T, Kozutsumi Y, Takematsu H, Kawasaki T, Suzuki A . Regulation of biosynthesis of N-glycolylneuraminic acid-containing glycoconjugates: characterization of factors required for NADH-dependent cytidine 5'monophosphate-N-acetylneuraminic acid hydroxylation. Glycoconj J. 1993; 10(1):109-15. DOI: 10.1007/BF00731194. View

18.

Varki A . Diversity in the sialic acids. Glycobiology. 1992; 2(1):25-40. PMC: 7108601. DOI: 10.1093/glycob/2.1.25. View

19.

Kozutsumi Y, Kawano T, Yamakawa T, Suzuki A . Participation of cytochrome b5 in CMP-N-acetylneuraminic acid hydroxylation in mouse liver cytosol. J Biochem. 1990; 108(5):704-6. DOI: 10.1093/oxfordjournals.jbchem.a123268. View

20.

Halliwell B, Gutteridge J . Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J. 1984; 219(1):1-14. PMC: 1153442. DOI: 10.1042/bj2190001. View