Comparative Studies on Bile Flow and Biliary Lipid Excretion After Bile-acid Loading in Normal and Partially Hepatectomized Rats

Overview

Journal Biochem J

Specialty Biochemistry

Date 1995 Jan 15

PMID 7832747

Citations 2

Authors

M Hoshino

A Hirano

T Hayakawa

Y Kamiya

T Ohiwa

A Tanaka

T Kumai

K Katagiri

M Miyaji

T Takeuchi

Affiliations

Soon will be listed here.

Abstract

This study was performed to investigate sequential changes in bile secretion and biliary lipids after taurocholic acid (TCA) loading of regenerating rat liver. TCA was administered intravenously at stepwise-increasing doses to groups of non-operated control and partially hepatectomized rats, 24, 72 and 168 h after surgery. Bile flow, bile-acid output (BAO) and phospholipid output (PLO) (expressed per gram of liver) in partially hepatectomized rats increased more than in the controls. Using an isolated perfusion rat-liver system, TCA infusion was also carried out on groups of non-operated control and hepatectomized rats 72 h after operation. Again bile flow, BAO and PLO (expressed per gram of liver) were significantly higher in the partial hepatectomy case, mirroring the results obtained in vivo. When horseradish peroxidase (HRP) was pulse-loaded in isolated perfusion preparations, the second peak of biliary HRP secretion in hepatectomized rats was significantly higher than in controls. We conclude that increased bile-acid flow in partially hepatectomized rats is dependent upon acceleration of vesicular transport accompanying or following proliferation in regenerating livers.

Citing Articles

Portal Hyperperfusion after Extended Hepatectomy Does Not Induce a Hepatic Arterial Buffer Response (HABR) but Impairs Mitochondrial Redox State and Hepatocellular Oxygenation.

Dold S, Richter S, Kollmar O, von Heesen M, Scheuer C, Laschke M PLoS One. 2015; 10(11):e0141877.

PMID: 26523932 PMC: 4629903. DOI: 10.1371/journal.pone.0141877.

Role of hepatic transporters in prevention of bile acid toxicity after partial hepatectomy in mice.

Csanaky I, Aleksunes L, Tanaka Y, Klaassen C Am J Physiol Gastrointest Liver Physiol. 2009; 297(3):G419-33.

PMID: 19497955 PMC: 2739828. DOI: 10.1152/ajpgi.90728.2008.

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