Studies on the Biosynthesis of Pancreatic Glucagon in the Pigeon (Columba Livia)

Overview

Journal Biochem J

Specialty Biochemistry

Date 1976 May 15

PMID 782450

Citations 4

Authors

K J OConnor

N R Lazarus

Affiliations

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Abstract

The biosynthesis of glucagon was studied in microdissected pigeon pancreatic, islets. [3H]-Tryotophan and [3H]leucine were incorporated into big and little glucagon. No precursor-product relationship was evident between big and little glucagon after radioactive pulsechase and immunoreactive chase incubations. Radioactive and immunoreactive little glucagon and immunoreactive big glucagon were actively secreted and the synthesis of both glucagons was inhibited by high concentrations of glucose. [3H]Tryptophan and [3H]leucine were incorporated into an islet protein of about 20000mol.wt. Gel filtration of extracts of turkey pancreas revealed the presence of an immunoreactive peak of mol.wt. approx. 20000. This glucagon-immunoreactive component was also present in dog and ox pancreas and was stable to chaotropic agents and elution at various pH values. A similar-sized glucagon-immunoreactive species was present in the dog circulation. These results are discussed in the light of the presently accepted mechanisms of glucagon biosynthesis.

Citing Articles

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Secretion by glucagonomas of a possible glucagon precursor.

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Dominant inheritance of large molecular weight immunoreactive glucagon.

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The purification and biological properties of pancreatic big glucagon.

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