O6-methylguanine-DNA Methyltransferase Activities in Biopsies of Human Melanoma Tumours

Overview

Journal Br J Cancer

Specialty Oncology

Date 1995 Jan 1

PMID 7819045

Citations 2

Authors

S Egyhazi

J Hansson

U Ringborg

Affiliations

Soon will be listed here.

Abstract

Tumour samples obtained from one primary melanoma and several lymph node and skin metastases were analysed for O6-methylguanine-DNA methyltransferase (MGMT) activity. While lymph node and skin metastases had similar average MGMT activity, the variance was significantly higher in lymph node metastases. Variability in MGMT activity was frequently observed in different metastases in the same individual and to a lesser extent within metastases.

Citing Articles

O6-methylguanine-DNA methyltransferase in pretreatment tumour biopsies as a predictor of response to temozolomide in melanoma.

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Systemic therapy of malignant melanoma.

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References

Karran P, Lindahl T, Griffin B . Adaptive response to alkylating agents involves alteration in situ of O6-methylguanine residues in DNA. Nature. 1979; 280(5717):76-7. DOI: 10.1038/280076a0. View

Comis R . DTIC (NSC-45388) in malignant melanoma: a perspective. Cancer Treat Rep. 1976; 60(2):165-76. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Karran P, Moscona A, Strauss B . Developmental decline in DNA repair in neural retina cells of chick embryos. Persistent deficiency of repair competence in a cell line derived from late embryos. J Cell Biol. 1977; 74(1):274-86. PMC: 2109876. DOI: 10.1083/jcb.74.1.274. View

Erickson L, Bradley M, Ducore J, Ewig R, Kohn K . DNA crosslinking and cytotoxicity in normal and transformed human cells treated with antitumor nitrosoureas. Proc Natl Acad Sci U S A. 1980; 77(1):467-71. PMC: 348292. DOI: 10.1073/pnas.77.1.467. View

Day 3rd R, Ziolkowski C, Scudiero D, Meyer S, Lubiniecki A, Girardi A . Defective repair of alkylated DNA by human tumour and SV40-transformed human cell strains. Nature. 1980; 288(5792):724-7. DOI: 10.1038/288724a0. View

Sklar R, Strauss B . Removal of O6-methylguanine from DNA of normal and xeroderma pigmentosum-derived lymphoblastoid lines. Nature. 1981; 289(5796):417-20. DOI: 10.1038/289417a0. View

Yarosh D, Foote R, Mitra S, Day 3rd R . Repair of O6-methylguanine in DNA by demethylation is lacking in Mer- human tumor cell strains. Carcinogenesis. 1983; 4(2):199-205. DOI: 10.1093/carcin/4.2.199. View

Myrnes B, Giercksky K, Krokan H . Interindividual variation in the activity of O6-methyl guanine-DNA methyltransferase and uracil-DNA glycosylase in human organs. Carcinogenesis. 1983; 4(12):1565-8. DOI: 10.1093/carcin/4.12.1565. View

10.

Wiestler O, Kleihues P, Pegg A . O6-alkylguanine-DNA alkyltransferase activity in human brain and brain tumors. Carcinogenesis. 1984; 5(1):121-4. DOI: 10.1093/carcin/5.1.121. View

11.

Scudiero D, Meyer S, Clatterbuck B, Mattern M, Ziolkowski C, Day 3rd R . Sensitivity of human cell strains having different abilities to repair O6-methylguanine in DNA to inactivation by alkylating agents including chloroethylnitrosoureas. Cancer Res. 1984; 44(6):2467-74. View

12.

Myrnes B, Norstrand K, Giercksky K, Sjunneskog C, Krokan H . A simplified assay for O6-methylguanine-DNA methyltransferase activity and its application to human neoplastic and non-neoplastic tissues. Carcinogenesis. 1984; 5(8):1061-4. DOI: 10.1093/carcin/5.8.1061. View

13.

Meer L, Janzer R, Kleihues P, KOLAR G . In vivo metabolism and reaction with DNA of the cytostatic agent, 5-(3,3-dimethyl-1-triazeno)imidazole-4-carboxamide (DTIC). Biochem Pharmacol. 1986; 35(19):3243-7. DOI: 10.1016/0006-2952(86)90419-3. View

14.

Fergusson R, Anderson L, Macpherson J, Robins P, Smyth J . Activity of a new nitrosourea (TCNU) in human lung cancer xenografts. Br J Cancer. 1988; 57(4):339-42. PMC: 2246564. DOI: 10.1038/bjc.1988.78. View

15.

Ringborg U, RUDENSTAM C, Hansson J, Hafstrom L, Stenstam B, Strander H . Dacarbazine versus dacarbazine-vindesine in disseminated malignant melanoma: a randomized phase II study. Med Oncol Tumor Pharmacother. 1989; 6(4):285-9. DOI: 10.1007/BF02985163. View

16.

Citron M, Decker R, Chen S, Schneider S, Graver M, Kleynerman L . O6-methylguanine-DNA methyltransferase in human normal and tumor tissue from brain, lung, and ovary. Cancer Res. 1991; 51(16):4131-4. View

17.

Cao E, Fan X, Yuan X, Xin S, Liu Y, Yu H . Levels of O6-methylguanine acceptor protein in extracts of human breast tumor tissues. Cancer Biochem Biophys. 1991; 12(1):53-8. View

18.

Egyhazi S, Bergh J, Hansson J, Karran P, Ringborg U . Carmustine-induced toxicity, DNA crosslinking and O6-methylguanine-DNA methyltransferase activity in two human lung cancer cell lines. Eur J Cancer. 1991; 27(12):1658-62. DOI: 10.1016/0277-5379(91)90440-o. View

19.

Lee S, RAFFERTY J, Elder R, Fan C, Bromley M, Harris M . Immunohistological examination of the inter- and intracellular distribution of O6-alkylguanine DNA-alkyltransferase in human liver and melanoma. Br J Cancer. 1992; 66(2):355-60. PMC: 1977793. DOI: 10.1038/bjc.1992.270. View

20.

Mineura K, Izumi I, Kuwahara N, Kowada M . O6-methylguanine-DNA methyltransferase activity in cerebral gliomas. A guidance for nitrosourea treatment?. Acta Oncol. 1994; 33(1):29-32. DOI: 10.3109/02841869409098371. View