Molecular Cloning of the Arabidopsis Thaliana Sedoheptulose-1,7-biphosphatase Gene and Expression Studies in Wheat and Arabidopsis Thaliana

Overview

Journal Plant Mol Biol

Date 1994 Nov 1

PMID 7811976

Citations 10

Authors

N M Willingham

J C Lloyd

C A Raines

Affiliations

Soon will be listed here.

Abstract

We report here the isolation and nucleotide sequence of genomic clones encoding the chloroplast enzyme sedoheptulose-1,7-bisphosphatase (SBPase) from Arabidopsis thaliana. The coding region of this gene contains eight exons (72-76 bp) and seven introns (75-91 bp) and encodes a polypeptide of 393 amino acids. Unusually, the 5' non-coding region contains two additional AUG codons upstream of the translation initiation codon. A comparison of the deduced Arabidopsis and wheat SBPase polypeptide sequences reveals 78.6%, identity. Expression studies showed that the level of SBPase mRNA in Arabidopsis and wheat is regulated in a light-dependent manner and is also influenced by the developmental stage of the leaf. Although the Arabidopsis SBPase gene is present in a single copy, two hybridizing transcripts were detected in some tissues, suggesting the presence of alternate transcription start sites in the upstream region.

Citing Articles

Sequence Characteristics and Expression Analysis of the Gene Encoding Sedoheptulose-1,7-Bisphosphatase, an Important Calvin Cycle Enzyme in Upland Cotton ( L.).

Chao M, Hu G, Dong J, Chen Y, Fu Y, Zhang J Int J Mol Sci. 2023; 24(7).

PMID: 37047620 PMC: 10095544. DOI: 10.3390/ijms24076648.

A single nucleotide substitution at the 3'-end of SBPase gene involved in Calvin cycle severely affects plant growth and grain yield in rice.

Li C, Li N, Huang R, Chen C, Guo J, Yang X BMC Plant Biol. 2020; 20(1):345.

PMID: 32698774 PMC: 7374905. DOI: 10.1186/s12870-020-02541-x.

Identification of Leaf Promoters for Use in Transgenic Wheat.

Alotaibi S, Sparks C, Parry M, Simkin A, Raines C Plants (Basel). 2018; 7(2).

PMID: 29597282 PMC: 6027260. DOI: 10.3390/plants7020027.

The ATG autophagic conjugation system in maize: ATG transcripts and abundance of the ATG8-lipid adduct are regulated by development and nutrient availability.

Chung T, Suttangkakul A, Vierstra R Plant Physiol. 2008; 149(1):220-34.

PMID: 18790996 PMC: 2613746. DOI: 10.1104/pp.108.126714.

Increased sedoheptulose-1,7-bisphosphatase activity in transgenic tobacco plants stimulates photosynthesis and growth from an early stage in development.

Lefebvre S, Lawson T, Zakhleniuk O, Lloyd J, Raines C, Fryer M Plant Physiol. 2005; 138(1):451-60.

PMID: 15863701 PMC: 1104198. DOI: 10.1104/pp.104.055046.

References

Mueller P, Hinnebusch A . Multiple upstream AUG codons mediate translational control of GCN4. Cell. 1986; 45(2):201-7. DOI: 10.1016/0092-8674(86)90384-3. View

FOX B, WALSH C . Mercuric reductase: homology to glutathione reductase and lipoamide dehydrogenase. Iodoacetamide alkylation and sequence of the active site peptide. Biochemistry. 1983; 22(17):4082-8. DOI: 10.1021/bi00286a014. View

Pruitt R, Meyerowitz E . Characterization of the genome of Arabidopsis thaliana. J Mol Biol. 1986; 187(2):169-83. DOI: 10.1016/0022-2836(86)90226-3. View

Gallagher T, Ellis R . Light-stimulated transcription of genes for two chloroplast polypeptides in isolated pea leaf nuclei. EMBO J. 1982; 1(12):1493-8. PMC: 553241. DOI: 10.1002/j.1460-2075.1982.tb01345.x. View

Lin A, Ashley G, Stubbe J . Location of the redox-active thiols of ribonucleotide reductase: sequence similarity between the Escherichia coli and Lactobacillus leichmannii enzymes. Biochemistry. 1987; 26(22):6905-9. DOI: 10.1021/bi00396a006. View

Murphy G, Kavanagh T . Speeding-up the sequencing of double-stranded DNA. Nucleic Acids Res. 1988; 16(11):5198. PMC: 336734. DOI: 10.1093/nar/16.11.5198. View

Lloyd J, Horsnell P, Dyer T, Raines C . Structure and sequence of a wheat phosphoribulokinase gene. Plant Mol Biol. 1991; 17(1):167-8. DOI: 10.1007/BF00036823. View

Kay R, Chan A, Daly M, McPherson J . Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes. Science. 1987; 236(4806):1299-302. DOI: 10.1126/science.236.4806.1299. View

Keegstra K, Bauerle C . Targeting of proteins into chloroplasts. Bioessays. 1988; 9(1):15-9. DOI: 10.1002/bies.950090105. View

10.

von Heijne G, Steppuhn J, Herrmann R . Domain structure of mitochondrial and chloroplast targeting peptides. Eur J Biochem. 1989; 180(3):535-45. DOI: 10.1111/j.1432-1033.1989.tb14679.x. View

11.

Baulcombe D, Buffard D . Gibberellic-acid-regulated expression of α-amylase and six other genes in wheat aleurone layers. Planta. 2013; 157(6):493-501. DOI: 10.1007/BF00396879. View

12.

Cadet F, Meunier J, Ferte N . Isolation and purification of chloroplastic spinach (Spinacia oleracea) sedoheptulose-1,7-bisphosphatase. Biochem J. 1987; 241(1):71-4. PMC: 1147525. DOI: 10.1042/bj2410071. View

13.

Lloyd J, Raines C, John U, Dyer T . The chloroplast FBPase gene of wheat: structure and expression of the promoter in photosynthetic and meristematic cells of transgenic tobacco plants. Mol Gen Genet. 1991; 225(2):209-16. DOI: 10.1007/BF00269850. View

14.

Shih M, Goodman H . Differential light regulated expression of nuclear genes encoding chloroplast and cytosolic glyceraldehyde-3-phosphate dehydrogenase in Nicotiana tabacum. EMBO J. 1988; 7(4):893-8. PMC: 454413. DOI: 10.1002/j.1460-2075.1988.tb02893.x. View

15.

Silverthorne J, Tobin E . Demonstration of transcriptional regulation of specific genes by phytochrome action. Proc Natl Acad Sci U S A. 1984; 81(4):1112-6. PMC: 344775. DOI: 10.1073/pnas.81.4.1112. View

16.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

17.

Raines C, Lloyd J, Dyer T . Molecular biology of the C3 photosynthetic carbon reduction cycle. Photosynth Res. 2014; 27(1):1-14. DOI: 10.1007/BF00029971. View

18.

Raines C, Lloyd J, Willingham N, Potts S, Dyer T . cDNA and gene sequences of wheat chloroplast sedoheptulose-1,7-bisphosphatase reveal homology with fructose-1,6-bisphosphatases. Eur J Biochem. 1992; 205(3):1053-9. DOI: 10.1111/j.1432-1033.1992.tb16873.x. View

19.

Henikoff S . Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984; 28(3):351-9. DOI: 10.1016/0378-1119(84)90153-7. View

20.

Raines C, Longstaff M, Lloyd J, Dyer T . Complete coding sequence of wheat phosphoribulokinase: developmental and light-dependent expression of the mRNA. Mol Gen Genet. 1989; 220(1):43-8. View