Cloning of Organic Solvent Tolerance Gene OstA That Determines N-hexane Tolerance Level in Escherichia Coli

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1994 Dec 1

PMID 7811102

Citations 14

Authors

R Aono

T Negishi

H Nakajima

Affiliations

Soon will be listed here.

Abstract

A variety of genes are involved in determining the level of organic solvent tolerance of Escherichia coli K-12. Gene ostA is one of the genes contributing to the level of organic solvent tolerance. This gene was cloned from an n-hexane-tolerant strain of E. coli, JA300. A JA300-based n-hexane-sensitive strain, OST4251, was converted to the n-hexane-tolerant phenotype by transformation with DNA containing the ostA gene derived from JA300. Thus, the cloned ostA gene complemented the n-hexane-sensitive phenotype of OST4251.

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